12 Awọn oriṣi ti Ẹjẹ atẹgun
Orukọ ọja
HWTS-RT071A 12 Awọn oriṣi ti Ohun elo Iwari Atẹgun Nucleic Acid (Fluorescence PCR)
ikanni
| ikanni | hu12Idaduro Idahun A | hu12Idaduro Idahun B | hu12Idaduro Idahun C | hu12Idaduro Idahun D |
| FAM | SARS-CoV-2 | HADV | HPIV Ⅰ | HRV |
| VIC/HEX | Iṣakoso ti abẹnu | Iṣakoso ti abẹnu | HPIV Ⅱ | Iṣakoso ti abẹnu |
| CY5 | IFV A | MP | HPIV Ⅲ | / |
| ROX | IFV B | RSV | HPIV | HMPV |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | SARS-CoV-2: 300 idaako/mlaarun ayọkẹlẹ B kokoro: 500 idaako/mlaarun ayọkẹlẹ A: 500 idaako/ml Adenovirus: 500 idaako/ml mycoplasma pneumoniae: 500 idaako/ml ọlọjẹ syncytial ti atẹgun: 500 idaako/ml, kokoro parainfluenza (Ⅰ, Ⅱ, Ⅲ, Ⅳ)：500 idaako/ml Rhinovirus: 500 idaako/ml eniyan metapneumovirus: 500 idaako/ml |
| Ni pato | Iwadii ifasilẹ-agbelebu fihan pe ko si ifasilẹ-agbelebu laarin ohun elo yii ati ọlọjẹ enterovirus A, B, C, D, ọlọjẹ epstein-barr, ọlọjẹ measles, cytomegalovirus eniyan, rotavirus, norovirus, ọlọjẹ mumps, ọlọjẹ varicella-herpes zoster, bordetella pertussis, streptococcus pyogenes, mycobacterium iko, aspergillus fumigatus, candida albicans, candida glabrata, pneumocystis jirovecii, cryptococcus neoformans ati eda eniyan genomic nucleic acid. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7500 Yara Real-Time PCR SystemsQuantStudio®5 Real-Time PCR Systems Awọn ọna PCR akoko-gidi SLAN-96P(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Eto PCR gidi-akoko, LineGene 9600 Plus Eto Wiwa PCR gidi-akoko (FQD-96A, imọ-ẹrọ Hangzhou Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1.
Niyanju isediwon reagent: Makiro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8) nipa Jiangsu Makiro & Micro-Test Med-Tech Co., Ltd., Isediwon yẹ ki o ṣee ṣe ni ibamu pẹlu awọn ilana.
Aṣayan 2.
Niyanju isediwon reagent: Makiro & Micro-Idanwo Gbogbogbo DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ati Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor ( HWTS-3006C, HWTS-3006B) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., isediwon yẹ ki o waiye ni ibamu si awọn ilana muna.Iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
Aṣayan 3.
Reagent isediwon ti a ṣeduro: Iyọkuro Acid Nucleic tabi Apo Isọdipo (YDP315) nipasẹ Tiangen Biotech(Beijing) Co., Ltd., isediwon yẹ ki o ṣe ni ibamu si awọn ilana ni muna.Iwọn didun elution ti a ṣe iṣeduro jẹ 100μL.
