Awọn oriṣi 17 ti HPV (16/18/6/11/44 Titẹ)
Orukọ ọja
HWTS-CC015 17 Awọn oriṣi ti Eda eniyan Papillomavirus (16/18/6/11/44 Titẹ) Ohun elo Iwari Acid Nucleic (Fluorescence PCR)
Arun-arun
Akàn jẹjẹ ọkan ninu awọn èèmọ buburu ti o wọpọ julọ ni apa ibisi obinrin.A ti fi han pe akoran HPV ti o tẹsiwaju ati awọn akoran pupọ jẹ ọkan ninu awọn idi pataki ti akàn cervical.Lọwọlọwọ aini awọn itọju imunadoko gbogbogbo tun wa fun alakan cervical ti o fa nipasẹ HPV.Nitoribẹẹ, wiwa ni kutukutu ati idena ti akoran ti oyun ti o fa nipasẹ HPV jẹ awọn bọtini si idena ti aarun alakan cervical.Idasile ti o rọrun, pato ati awọn idanwo iwadii aisan iyara fun awọn pathogens jẹ pataki nla fun iwadii ile-iwosan ti akàn cervical.
ikanni
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Iṣakoso inu |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Iṣakoso inu |
Imọ paramita
| Ibi ipamọ | -18 ℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Apeere ito, Apeere swab ti inu obinrin, Apeere swab abo abo |
| Ct | ≤28 |
| LoD | 300 idaako/ml |
| Ni pato | Ko si ifaseyin-agbelebu pẹlu Ureaplasma urealyticum, Chlamydia trachomatis ti ibisi ibisi, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold , Gardnerella ati awọn iru HPV miiran ti ko ni aabo nipasẹ ohun elo naa. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Awọn ọna Iwari PCR akoko-gidi MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Niyanju isediwon reagent: Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Igbeyewo. Laifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Fi 200μL ti saline deede lati tun pada pellet ni igbese 2.1, ati lẹhinna isediwon yẹ ki o wa ni ibamu pẹlu si awọn ilana fun lilo ti yi reagent isediwon.Iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
Niyanju isediwon reagent: QIAamp DNA Kit Mini Kit (51304) tabi Makiro & Micro-Test Viral DNA/RNA Ọwọn (HWTS-3020-50).Ṣafikun 200μL ti iyọ deede lati tun pada pellet ni igbese 2.1, ati lẹhinna isediwon yẹ ki o waiye ni ibamu si awọn ilana fun lilo reagent isediwon yii.Iwọn ayẹwo ti awọn ayẹwo jẹ gbogbo 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 100μL.
Niyanju isediwon reagent: Makiro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L).Ṣafikun 200μL ti reagent itusilẹ ayẹwo lati tun da pellet pada ni igbesẹ 2.1, ati lẹhinna isediwon yẹ ki o ṣe ni ibamu si awọn ilana fun lilo reagent isediwon yii.
