4 Iru awọn ọlọjẹ atẹgun
Orukọ ọja
HWTS-RT099-4 Awọn oriṣi ti Awọn ọlọjẹ Atẹmi Apo Iwari Acid Nucleic Acid (Fluorescence PCR)
Arun-arun
Arun Iwoye Corona 2019, tọka si bi “COVID-19”, tọka si ẹdọforo ti o fa nipasẹ2019-nCoVàkóràn.2019-nCoVjẹ coronavirus ti o jẹ ti iwin β.COVID-19 jẹ arun ajakalẹ-arun ti atẹgun nla, ati pe olugbe ni ifaragba gbogbogbo.Lọwọlọwọ, orisun ti akoran jẹ pataki awọn alaisan ti o ni akoran nipasẹ2019-nCoV, ati awọn eniyan ti o ni asymptomatic le tun di orisun ti akoran.Da lori iwadii ajakale-arun lọwọlọwọ, akoko isubu jẹ awọn ọjọ 1-14, pupọ julọ awọn ọjọ 3-7.Iba, Ikọaláìdúró gbigbẹ ati rirẹ jẹ awọn ifarahan akọkọ.Awọn alaisan diẹ ni awọn aami aisans biiimu imu, imu imu, ọfun ọfun, myalgia ati gbuuru, ati be be lo.
ikanni
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | -18 ℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Awọn ẹda / milimitaKokoro aarun ayọkẹlẹ A / Kokoro aarun ayọkẹlẹ B / Kokoro syncytial ti atẹgun: 500 Awọn ẹda/mL |
| Ni pato | a) Awọn abajade ifaseyin agbekọja fihan pe ko si ifaseyin agbelebu laarin ohun elo ati coronavirus eniyan SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, ọlọjẹ parainfluenza 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus eniyan, enterovirus A, B, C, D, kokoro ẹdọforo eniyan, kokoro epstein-barr, kokoro measles, kokoro cytomegalo eniyan, rotavirus, norovirus, virus parotitis, varicella-zoster kokoro, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium iko, ẹfin aspergillus, candida albicans, candidanemic pneumonia acid titun, . b) Agbara kikọlu: yan mucin (60mg/mL), 10% (v/v) ti ẹjẹ ati phenylephrine (2mg/mL), oxymetazoline (2mg/mL), iṣuu soda kiloraidi (pẹlu awọn olutọju) (20 mg/mL) ), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL). ), histamini hydrochloride (5mg/mL), alpha interferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/ml), oseltamivir (60ng/ml), peramivir (1mg/mL), lopinavir (500mg/ milimita Ritonavir (60mg/ml), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/ml), meropenem (200mg/mL), levofloxacin (10μg/ml) ati tobramycin (0.6mg/mL) mL) fun idanwo kikọlu, ati awọn abajade fihan pe awọn nkan kikọlu pẹlu awọn ifọkansi ti a mẹnuba loke ko ni esi kikọlu si awọn abajade idanwo ti awọn ọlọjẹ. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems |
Sisan iṣẹ
Aṣayan 1.
Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006) ti a ṣe nipasẹ Jiangsu Macro & Micro -Test Med-Tech Co., Ltd. Iwọn ayẹwo ti a ti jade jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
Aṣayan 2.
QIAamp Viral RNA Mini Kit (52904) ti a ṣe nipasẹ QIAGEN tabi Nucleic Acid Extraction or Purification Kit (YDP315-R) ti a ṣe nipasẹ Tiangen Biotech (Beijing) Co., Ltd. Iwọn ayẹwo ti o jade jẹ 140μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 60μL.
