4 Iru awọn ọlọjẹ atẹgun
Orukọ ọja
HWTS-RT099- Awọn oriṣi mẹrin ti Awọn ọlọjẹ Atẹmi Apo Iwari Acid Nucleic Acid (Pluorescence PCR)-内参是NED-ABI 7500 Eto PCR-gidi/ABI 7500 Yara Real-Time PCR Systems / QuantStudio®5 Real-Time PCR Systems
HWTS-RT158-4 Awọn oriṣi ti Awọn ọlọjẹ Atẹmi Apo Iwari Acid Nucleic Acid (Fluorescence PCR) -Quasar 705
Arun-arun
Arun Iwoye Corona 2019, tọka si bi “COVID-19”, tọka si ẹdọforo ti o fa nipasẹ2019-nCoVàkóràn.2019-nCoVjẹ coronavirus ti o jẹ ti iwin β. COVID-19 jẹ arun ajakalẹ-arun ti atẹgun nla, ati pe olugbe ni ifaragba gbogbogbo. Lọwọlọwọ, orisun ti akoran jẹ pataki awọn alaisan ti o ni akoran nipasẹ2019-nCoV, ati awọn eniyan ti o ni asymptomatic le tun di orisun ti akoran. Da lori iwadii ajakale-arun lọwọlọwọ, akoko isubu jẹ awọn ọjọ 1-14, pupọ julọ awọn ọjọ 3-7. Iba, Ikọaláìdúró gbigbẹ ati rirẹ jẹ awọn ifarahan akọkọ. Awọn alaisan diẹ ni awọn aami aisans biiimu imu, imu imu, ọfun ọfun, myalgia ati gbuuru, ati be be lo.
ikanni
| FAM | Ọdun 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | -18 ℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Awọn ẹda / milimitaKokoro aarun ayọkẹlẹ A / Kokoro aarun ayọkẹlẹ B / Kokoro syncytial ti atẹgun: 500 Awọn ẹda/mL |
| Ni pato | a) Awọn abajade ifaseyin agbekọja fihan pe ko si ifaseyin agbelebu laarin ohun elo ati coronavirus eniyan SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, ọlọjẹ parainfluenza iru 1, 2, 3, rhinovirus A, B,virus eniyan, chulamyvirus A, B, C. enterovirus A, B, C, D, ọlọjẹ ẹdọforo eniyan, ọlọjẹ epstein-barr, ọlọjẹ measles, ọlọjẹ cytomegalo eniyan, rotavirus, norovirus, virus parotitis, virus varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aures, strepstreuptococcus aureus, pstreuptooges klebsiella pneumoniae, mycobacterium tuberculosis, ẹfin aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci ati cryptococcus ọmọ ikoko ati eda eniyan jenomic nucleic acid. b) Agbara kikọlu: yan mucin (60mg / mL), 10% (v / v) ti ẹjẹ ati phenylephrine (2mg / mL), oxymetazoline (2mg / milimita), iṣuu soda kiloraidi (pẹlu awọn olutọju) (20 mg / mL), beclomethasone (20mg / mL), 20mgolimde, dexamnisethasone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/ml), zanamivir (20mg/ml0), ogidimivir (20mg/ml0), opim ribavirin (60ng/ml), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/ml), meropenem (200mg/mL), toflocin (200mg/mL), toflocin (0.6mg / mL) fun idanwo kikọlu, ati awọn abajade fihan pe awọn nkan kikọlu pẹlu awọn ifọkansi ti a mẹnuba loke ko ni idahun kikọlu si awọn abajade idanwo ti awọn ọlọjẹ. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR SystemApplied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems |
Sisan iṣẹ
Aṣayan 1.
Makiro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) ti a ṣe nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. iwọn didun jẹ 80 μl.
Aṣayan 2.
QIAamp Viral RNA Mini Kit (52904) ti a ṣe nipasẹ QIAGEN tabi Nucleic Acid Extraction or Purification Kit (YDP315-R) ti a ṣe nipasẹ Tiangen Biotech (Beijing) Co., Ltd. Iwọn ayẹwo ti o jade jẹ 140μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 60μL.
