Adenovirus Agbaye
Orukọ ọja
HWTS-RT017A Adenovirus Ohun elo Iwari Acid Nucleic Acid Agbaye (Fluorescence PCR)
Arun-arun
Adenovirus eniyan (HAdV) jẹ ti iwin adenovirus Mammalian, eyiti o jẹ ọlọjẹ DNA ti o ni ilopo meji laisi apoowe.Awọn adenoviruses ti a ti rii bẹ pẹlu awọn ẹgbẹ-ẹgbẹ 7 (AG) ati awọn oriṣi 67, eyiti 55 serotypes jẹ ọlọjẹ si eniyan.Lara wọn, o le ja si awọn akoran atẹgun atẹgun jẹ akọkọ ẹgbẹ B (Awọn oriṣi 3, 7, 11, 14, 16, 21, 50, 55), Ẹgbẹ C (Awọn oriṣi 1, 2, 5, 6, 57) ati Ẹgbẹ E (Iru 4), ati pe o le ja si ikolu gbuuru ifun jẹ Ẹgbẹ F (Awọn oriṣi 40 ati 41) [1-8].Awọn oriṣi oriṣiriṣi ni awọn aami aisan ile-iwosan oriṣiriṣi, ṣugbọn paapaa awọn akoran ti atẹgun atẹgun.Awọn arun atẹgun ti o fa nipasẹ awọn akoran atẹgun atẹgun ti ara eniyan ni iroyin fun 5% ~ 15% ti awọn arun atẹgun agbaye, ati 5% -7% ti awọn arun atẹgun ọmọde agbaye[9].Adenovirus jẹ apanirun ni ọpọlọpọ awọn agbegbe ati pe o le ni akoran ni gbogbo ọdun yika, paapaa ni awọn agbegbe ti o kunju, eyiti o ni itara si awọn ibesile agbegbe, paapaa ni awọn ile-iwe ati awọn ibudo ologun.
ikanni
| FAM | adenovirus agbayenucleic acid |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Nasopharyngeal swab,Ọfun swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 idaako/ml |
| Ni pato | a) Ṣe idanwo awọn itọkasi odi odiwọn ile-iṣẹ nipasẹ ohun elo, ati abajade idanwo ni ibamu pẹlu awọn ibeere. b) Lo ohun elo yii lati ṣawari ati pe ko si ifasilẹ-agbelebu pẹlu awọn aarun atẹgun miiran (gẹgẹbi ọlọjẹ Aarun ayọkẹlẹ, ọlọjẹ Aarun ayọkẹlẹ B, ọlọjẹ syncytial ti atẹgun, ọlọjẹ Parainfluenza, Rhinovirus, Eniyan metapneumovirus, ati bẹbẹ lọ) tabi kokoro arun (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, ati bẹbẹ lọ). |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR gidi-akoko (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin Systems (FQD-96A, HangzhouImọ-ẹrọ Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Awọn ọna PCR-gidi-gidi, BioRad CFX Opus 96 Awọn ọna PCR-gidi-gidi |
Sisan iṣẹ
(1) Reagent isediwon ti a ṣe iṣeduro:Makiro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8).Awọn isediwon yẹ ki o wa ni ošišẹ ti ni ibamu si awọn ilana.Ayẹwo ti o jade ni awọn alaisan'nasopharyngeal swab tabi ọfun swab awọn ayẹwo ti a gba lori aaye.Ṣafikun awọn ayẹwo sinu reagent itusilẹ ayẹwo nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex lati dapọ daradara, gbe ni iwọn otutu yara fun awọn iṣẹju 5, mu jade lẹhinna yi pada ki o dapọ daradara lati gba DNA ti kọọkan ayẹwo.
(2) Niyanju reagenti isediwon:Makiro & Micro-igbeyewo Gbogun ti DNA/RNA Apo(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).isẹ naa yẹ ki o ṣe ni ibamu pẹlu awọn ilana.Iwọn ayẹwo ti o jade jẹ 200μL, ati awọnniyanju elution iwọn didunis80μL.
(3) Reagent isediwon ti a ṣe iṣeduro: Iyọkuro Acid Nucleic tabi Reagent Mimọ (YDP)315) nipasẹ Tiangen Biotech (Beijing) Co., Ltd., awọnisẹ yẹ ki o wa ni ošišẹ ti ni ibamu pẹlu awọn ilana.Iwọn ayẹwo ti o jade jẹ 200μL, ati awọnniyanju elution iwọn didunis80μL.
