ALDH Jiini Polymorphism
Orukọ ọja
HWTS-GE015ALDH Genetic Polymorphism Apo Iwari (ARMS -PCR)
Arun-arun
ALDH2 gene (acetaldehyde dehydrogenase 2), wa lori eda eniyan chromosome 12. ALDH2 ni esterase, dehydrogenase ati iṣẹ reductase ni akoko kanna. Awọn ẹkọ-ẹkọ ti fihan pe ALDH2 jẹ enzymu ti iṣelọpọ ti nitroglycerin, eyiti o ṣe iyipada nitroglycerin sinu ohun elo afẹfẹ nitric, nitorinaa isinmi awọn ohun elo ẹjẹ ati imudarasi awọn rudurudu sisan ẹjẹ. Sibẹsibẹ, awọn polymorphisms wa ninu jiini ALDH2, eyiti o dojukọ ni Ila-oorun Asia. Egan-iru ALDH2 * 1/* 1 GG ni agbara iṣelọpọ ti o lagbara, lakoko ti iru heterozygous ni 6% nikan ti iṣẹ-ṣiṣe enzymu-iru-ẹgan, ati iru mutant homozygous ni o ni fere iṣẹ-ṣiṣe enzymu odo odo, pẹlu iṣelọpọ agbara pupọ ati pe ko le ṣe aṣeyọri ipa ti o fẹ, nitorinaa nfa ipalara si ara eniyan.
ikanni
| FAM | ALDH2 |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Ẹjẹ anticoagulated EDTA |
| CV | <5.0 |
| LoD | 103Awọn ẹda/ml |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Niyanju isediwon reagents: lo Ẹjẹ Genome DNA isediwon Apo (DP318) nipa Tiangen Biotech (Beijing) Co., Ltd. tabi Apo Iyọkuro Jiini Ẹjẹ (A1120) nipasẹ Promega lati yọ EDTA ẹjẹ anticoagulated Genomic DNA jade.
Niyanju isediwon reagents: Makiro & Micro-Test Gbogbogbo DNA/RNA Apo (HWTS-3019) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Test Aifọwọyi Nucleic Acid Extractor (HWTS-EQ011)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Isediwon yẹ ki o waiye ni ibamu si awọn ilana ti o muna. Awọn niyanju elution iwọn didun ni100μL.
