Carbapeem Coronce Geneen (KPC / NDM / Ox 48 / OxA 23 / MIM / Map)
Orukọ ọja
Hwts-Ot045 Carbapeem resistance Hene (KPC / NDM / Oxa 28 / Ifojuu / Im)
Onimọraciology
Awọn egboogi-ara carbapene jẹ awọn egboogi-inimical xam pẹlu ohun elo ajesara ti o gbooro julọ ati iṣẹ ṣiṣe antibacterial ti o lagbara. Nitori iduroṣinṣin rẹ si β-lactamase ati majele kekere, o ti di ọkan ninu awọn oogun antibacterial pataki julọ fun itọju ti awọn akoran ti ko ni arun. Carbapenems jẹ iduroṣinṣin pupọ si plasmid-angitated gbooro-sprectump ati awọn chromosomes, ati plamomomes, ati awọn ensames Ampc.
Ikanni
| Pcr-dapọ 1 | PCR-Ijọpọ 2 | |
| Fa | Mamipi | Ewe-ọwọ |
| Vic / Hex | Iṣakoso inu | Iṣakoso inu |
| Gigunkini | Ndm | Kpc |
| Roox | OxA48
| Ox23 |
Awọn ohun elo imọ-ẹrọ
| Ibi ipamọ | ≤-18 ℃ |
| Oju aye | Oṣu mejila 12 |
| Iru apẹrẹ | Sputum, awọn ileto funfun, swab onigun |
| Ct | ≤3 |
| CV | ≤5.0% |
| Lto | 103Cfuye / milimita |
| Alaye pataki | A) Awọn ohun elo naa ṣawari awọn ile-iṣẹ odiwọn odiwọn, ati awọn abajade ba awọn ibeere ti awọn itọkasi ti o baamu. b) Awọn abajade ti idanwo-ifasijasi ti o han pe ohun elo yii ko ni agbara ti atẹgun miiran, pseudomonas aeugunisse, pyeudococcucus auremosis, staphytous, staphytous, staphytous, clebsielca oxytoca, HAEMOphulus fadzilluclucturact juii axanicusticustisticus, pludomonia coli, escychus coli, esperovirus ctx, meka, sma, Shv, temit, bbl c) Anti-inter: Mucin, Minicycline, Clatmiki, Cipromycin, Ciproflopnicon acid, chiprofloxne, ciprofloxon acid si iṣawari ti Carbapeem resistance awọn jiini KPC, ndm, ox48, ola23, vim, ati Imi. |
| Awọn ohun elo ti o wulo | Lo awọn biosystems 7500 awọn eto PC-akoko akoko-akoko Awọn eto PC-akoko akoko-akoko gidi Awọn eto PCR-96P gidi-akoko Ẹrọ Percyclercler®480 Eto PRC Linegene 9600 Plut eto iṣawari akoko-akoko PCR (FQD-96a,HanlzhouImọ-ẹrọ Bioer) Ma-6000 Giga iwọn-akoko giga-akoko giga-akoko (Suzhou Molarray Co., Ltd.) Eto PFX96 Akoko PRC Biokad CFX Opus 96 Eto PRC |
Iṣẹ sisan
Aṣayan 1.
Atọpa imukuro iṣeduro: Macro & Micro-Idanwo Lancense General DNA / RNE Kit (HWTS-3019-50, HWTS - 3019-32, HWTS-3019-48, HWTS-3019-96) (eyiti o le ṣee lo pẹlu macro & bulọọgi-Idanwo Idanwo Nuclenic Acid alaifọwọyi (HWTS-3006B) Apeyọri Thalkus. Awọn igbesẹ atẹle yẹ ki o tẹle awọn itọnisọna fun isediwon, ati iwọn lilo Ikojọpọ ti a ṣe iṣeduro ni100μL.
Aṣayan 2.
A ṣe iṣeduro iyọrisi iyọkuro ti a ṣe iṣeduro: isediwon meclenic tabi isọdọmọ , ati gbọn titi ti Thalpus ti fi idi idaduro patapata. Lo omi RNEASE / DNnase Omi ọfẹ Fun Elefin, ati Iwọn Iroro Ero ti ile-iwosan jẹ 100μL.
Aṣayan 3.
Iṣeduro isediwon isediwon: Macro & Idanwo Idanwo Idanwo Atilẹyin Ituwọrẹ. Awọn ayẹwo sputum nilo lati fo nipa fifi 1ml ti iyo ti o sọ tẹlẹ ni iṣaaju, ati pe o fun supernatant (pa supernatant). Fun awọn mimọ mimọ ati swalot funfun, ṣafikun 50μL taara si awọn igbesẹ ti a darukọ loke palkus pipe, ati pe o yẹ ki awọn igbesẹ atẹle ni ibamu si ilana fun lilo.
