Gene Resistance Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Orukọ ọja
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Ohun elo Iwari (Fluorescence PCR)
Arun-arun
Awọn aporo aarun ayọkẹlẹ Carbapenem jẹ apaniyan β-lactam aṣoju pẹlu irisi antibacterial ti o gbooro julọ ati iṣẹ ṣiṣe antibacterial ti o lagbara julọ.Nitori iduroṣinṣin rẹ si β-lactamase ati majele kekere, o ti di ọkan ninu awọn oogun antibacterial ti o ṣe pataki julọ fun itọju awọn akoran kokoro-arun nla.Awọn Carbapenems jẹ iduroṣinṣin to gaju si β-lactamases (ESBLs) ti o gbooro sii ti plasmid, awọn chromosomes, ati awọn cephalosporinases ti plasmid-mediated (awọn enzymu AmpC).
ikanni
| PCR-Idapọ 1 | PCR-Idapọ 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Iṣakoso ti abẹnu | Iṣakoso ti abẹnu |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Sputum, awọn ileto mimọ, swab rectal |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/ml |
| Ni pato | a) Ohun elo naa ṣe awari awọn itọkasi odi ile-iṣẹ ti o ni idiwọn, ati awọn abajade pade awọn ibeere ti awọn itọkasi ti o baamu. b) Awọn abajade ti idanwo ifasilẹ-agbelebu fihan pe kit yii ko ni ifasẹyin agbelebu pẹlu awọn aarun atẹgun miiran, gẹgẹbi Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Kleb junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, tabi awọn ayẹwo ti o ni awọn miiran ti oògùn-sooro Jiini CTX, mecA, SME, SHV. c) Anti-kikọlu: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin ti wa ni ti a ti yan fun kikọlu igbeyewo, ati awọn esi fihan wipe awọn loke-darukọ interfering oludoti ni ko si interfering. si wiwa awọn jiini resistance carbapenem KPC, NDM, OXA48, OXA23, VIM, ati IMP. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR gidi-akoko (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Eto Wiwa PCR akoko-gidi (FQD-96A,HangzhouImọ-ẹrọ Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1.
Niyanju isediwon reagent: Makiro & Micro-Idanwo Gbogbogbo DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (eyi ti o le ṣee lo pẹlu Macro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Fi 200μL ti iyọ deede si awọn thallus precipitate.Awọn igbesẹ ti o tẹle yẹ ki o tẹle awọn itọnisọna fun isediwon, ati iwọn didun elution ti a ṣe iṣeduro jẹ100μL.
Aṣayan 2.
Reagent isediwon ti a ṣe iṣeduro: Iyọkuro Acid Nucleic Acid tabi Reagent Iwẹnumọ (YDP302) nipasẹ Tiangen Biotech (Beijing) Co., Ltd. Iyọkuro yẹ ki o bẹrẹ ni ibamu pẹlu igbesẹ 2 ti itọnisọna fun lilo (fi 200μL ti ifipamọ GA si thallus precipitate). , ati gbigbọn titi ti thallus yoo fi daduro patapata).Lo omi ọfẹ RNase/DNase fun elution, ati iwọn didun elution ti o ni iyìn jẹ 100μL.
Aṣayan 3.
Reagenti isediwon ti a ṣe iṣeduro: Makiro & Micro-Test Ayẹwo Tu Reagent.Apeere sputum nilo lati fọ nipasẹ fifi 1mL ti iyọ deede kun si thallus precipitate ti a mẹnuba loke, ti a sọ ni centrifuged ni 13000r/min fun awọn iṣẹju 5, ati supernatant ti sọnu (pa 10-20µL ti supernatant mọ).Fun ileto mimọ ati swab rectal, ṣafikun 50μL ti reagent itusilẹ ayẹwo taara si thallus precipitate ti a mẹnuba loke, ati awọn igbesẹ ti o tẹle yẹ ki o fa jade ni ibamu si itọnisọna fun lilo.
