Coxsackie Iwoye Iru A16 Nucleic Acid
Orukọ ọja
HWTS-EV025-Coxsackie Iwoye Iru A16 Apo Iwari Acid Nucleic Acid (Amudara Isothermal Probe Enzymatic)
Iwe-ẹri
CE
Arun-arun
Ohun elo yii gba Enzymatic Probe Isothermal Amplification (EPIA), ati ṣe apẹrẹ awọn alakoko kan pato ati awọn iwadii ti o ni ipilẹ RNA (rProbe) fun agbegbe ti o ni aabo pupọ ti Cox A16, ati ṣafikun enzymu Bst ati enzyme RNaseH ni akoko kanna, ninu eyiti osi ati Awọn opin ọtun ti ipilẹ RNA ti rProbe jẹ aami pẹlu awọn ẹgbẹ fluorescent ati quencher, lẹsẹsẹ.Lo iṣẹ ṣiṣe DNA polymerase ati iṣẹ iṣipopada okun ti enzymu Bst lati mu ibi-afẹde pọ si lati ṣe idanwo ni iwọn otutu igbagbogbo, enzymu RNaseH le ya awọn ipilẹ RNA lori pq arabara ibi-afẹde, ki ẹgbẹ fluorescent ati quencher ti rProbe jẹ niya nipa fluorescing.Ni afikun, ajẹkù osi ti ipilẹ rProbe RNA ti o ku le ṣee lo bi alakoko lati fa siwaju sii lati dagba ọja kan, eyiti o ṣajọpọ ọja naa siwaju.Ifihan agbara Fuluorisenti ti wa ni ikojọpọ nigbagbogbo pẹlu dida ọja naa, nitorinaa riri wiwa ti acid nucleic afojusun.
ikanni
FAM | Coxsackie kokoro iru A16 |
ROX | ti abẹnu Iṣakoso |
Imọ paramita
Ibi ipamọ | ≤-18℃ |
Selifu-aye | 12 osu |
Apeere Iru | Titun gba ọfun swabs |
CV | ≤10.0% |
Ct | ≤38 |
LoD | 2000 Awọn ẹda/ml |
Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR SystemsSLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto Eto Wiwa Iwọn otutu Ibakan ti Fluorescence gidi-gidi Rọrun Amp HWTS1600 |
Sisan iṣẹ
Aṣayan 1
Niyanju isediwon reagent: Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) ati Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor (HWTS-3006)
Aṣayan 2
Niyanju isediwon reagent: Makiro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8).