Enterovirus Universal, EV71 ati CoxA16
Orukọ ọja
HWTS-EV026B-Enterovirus Universal, EV71 ati CoxA16 Ohun elo Iwari Acid Nucleic (Fluorescence PCR)
HWTS-EV020Y/Z-Didi-si dahùn o Enterovirus Universal, EV71 ati CoxA16 Nucleic Acid Apo (Fluorescence PCR)
Iwe-ẹri
CE/MDA (HWTS-EV026)
Arun-arun
Arun-ẹnu-ọwọ (HFMD) jẹ arun ajakalẹ-arun ti o wọpọ ni awọn ọmọde. O maa nwaye ninu awọn ọmọde labẹ ọdun 5, ati pe o le fa awọn herpes ni ọwọ, ẹsẹ, ẹnu ati awọn ẹya miiran, ati pe nọmba kekere ti awọn ọmọde le fa awọn ilolu bii myocarditis, edema ẹdọforo, aseptic meningoencephalitis, bbl.
Lọwọlọwọ, a ti ri 108 serotypes ti enteroviruses, eyiti o pin si awọn ẹgbẹ mẹrin: A, B, C ati D. Enteroviruses ti o fa HFMD jẹ oriṣiriṣi, ṣugbọn enterovirus 71 (EV71) ati coxsackievirus A16 (CoxA16) jẹ eyiti o wọpọ julọ ati ni afikun si HFMD, o le fa ipalara ti aarin-ara ti o lagbara, paramisi ti o niiṣe pẹlu paracciitis.
ikanni
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Iṣakoso inu |
Imọ paramita
| Ibi ipamọ | Omi: ≤-18℃ Ninu okunkunLyophilization: ≤30℃ |
| Selifu-aye | Omi: 9 osuLyophilization: 12 osu |
| Apeere Iru | Ayẹwo swab ọfun, ito Herpes |
| Ct | ≤38 |
| CV | ≤5.0 |
| LoD | 500 idaako/ml |
| Awọn ohun elo ti o wulo | O le baramu awọn ohun elo PCR Fuluorisenti akọkọ lori ọja naa.ABI 7500 Real-Time PCR Systems ABI 7500 Yara Real-Time PCR Systems SLAN-96P Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Awọn ọna Iwari PCR akoko-gidi MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Lapapọ PCR Solusan
Niyanju isediwon reagent: Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Test Aifọwọyi Nucleic Acid Extractor (HWTS-EQ011)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Awọn isediwon yẹ ki o wa ni ti gbe jade ni ibamu si. Iwọn ayẹwo ti o jade jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
Niyanju isediwon reagent: Makiro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8). Isediwon yẹ ki o ṣe ni ibamu si itọnisọna itọnisọna. Awọn ayẹwo isediwon jẹ swabs oropharyngeal tabi awọn ayẹwo ito Herpes ti awọn alaisan eyiti o gba lori aaye. Ṣafikun awọn swabs ti a gba taara taara si Macro & Micro-Test Ayẹwo Tu Reagent, vortex ati dapọ daradara, gbe ni iwọn otutu yara fun awọn iṣẹju 5, mu jade lẹhinna yi pada ki o dapọ daradara lati gba RNA ti ayẹwo kọọkan.
Aṣeyọri isediwon ti a ṣe iṣeduro: QIAamp Viral RNA Mini Kit (52904) nipasẹ QIAGEN tabi Iyọkuro Acid Nucleic tabi Reagent Mimọ (YDP315-R). Isediwon yẹ ki o ṣe ni ibamu pẹlu ilana itọnisọna.
