Igbo Encephalitis Iwoye
Orukọ ọja
HWTS-FE006 Igbo Encephalitis Iwoye Ohun elo Iwari Acid Acid (Fluorescence PCR)
Arun-arun
Encephalitis igbo (FE), ti a tun mọ si encephalitis tick-borne (Encephalitis Tick-borne, TBE), jẹ arun ajakalẹ-arun ti eto aifọkanbalẹ aarin ti o fa nipasẹ ọlọjẹ encephalitis igbo. Kokoro encephalitis igbo jẹ ti iwin Flavivirus ti idile Flaviviridae. Awọn patikulu ọlọjẹ jẹ iyipo pẹlu iwọn ila opin ti 40-50nm. Iwọn molikula jẹ nipa 4×106Bẹẹni, ati jiini ọlọjẹ jẹ oye-daadaa, RNA ti o ni okun kan[1]. Ni ile-iwosan, o jẹ ifihan nipasẹ iba giga, orififo, coma, ibẹrẹ iyara ti irritation meningeal, ati paralysis iṣan ti ọrun ati awọn ẹsẹ, ati pe o ni oṣuwọn iku ti o ga. Ṣiṣayẹwo ni kutukutu ati iyara ti kokoro encephalitis igbo jẹ bọtini si itọju ti encephalitis igbo, ati idasile ọna ti o rọrun, pato ati ọna iwadii etiological ti o yara jẹ pataki pupọ ninu iwadii ile-iwosan ti encephalitis igbo.[1,2].
ikanni
| FAM | kokoro encephalitis igbo nucleic acid |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | omi ara tuntun |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 idaako/ml |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Awọn ọna Iwari PCR akoko-gidi MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Reagent isediwon ti a ṣeduro: Iyọkuro Acid Nucleic tabi Apo Isọdipo (YDP315-R) nipasẹ Tiangen Biotech(Beijing) Co., Ltd., isediwon yẹ ki o ṣe ni ibamu si awọn itọnisọna muna. Iwọn iwọn ayẹwo ti a ṣe iṣeduro jẹ 140μL ati iwọn didun elution ti a ṣe iṣeduro jẹ 60μL.
Niyanju isediwon reagent: Makiro & Micro-Idanwo Gbogbogbo DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ati Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor (HWTS-6W0-300). HWTS-3006B). isediwon yẹ ki o wa ni o waiye ni ibamu si awọn ilana muna. Iwọn iwọn ayẹwo ti a ṣe iṣeduro jẹ 200μL ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
