Hepatitis B Virus DNA Quantitative Fluorescence
Orukọ ọja
HWTS-HP015 Hepatitis B Virus DNA Quantitative Fluorescence Ayẹwo Apo (Fluorescence PCR)
Arun-arun
Hepatitis B jẹ arun ti o fa nipasẹ ọlọjẹ jedojedo B (HBV), eyiti o jẹ afihan nipasẹ awọn egbo iredodo ẹdọ, ati pe o le fa ibajẹ awọn ẹya ara pupọ. Awọn alaisan jedojedo B ni a ṣe afihan ni ile-iwosan bi rirẹ, isonu ti ounjẹ, apa isalẹ tabi edema gbogbogbo, ati ẹdọforo nitori iṣẹ ẹdọ ti bajẹ. Ida marun ninu ọgọrun ti awọn agbalagba ti o ni akoran ati 95% ti awọn eniyan ti o ni inaro ko le mu HBV kuro ni imunadoko, ti o yọrisi ikolu ọlọjẹ ti o tẹsiwaju, ati diẹ ninu awọn akoran onibaje bajẹ di cirrhosis ẹdọ ati carcinoma hepatocellular.[1-4].
ikanni
| FAM | HBV-DNA |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | omi ara tuntun, pilasima |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 5 IU/ml |
| Ni pato | Awọn abajade pato fihan pe gbogbo awọn ọran 50 ti ilera HBV DNA ti o ni ilera awọn ayẹwo omi ara jẹ odi; awọn abajade idanwo ifasilẹ-agbelebu fihan pe ko si ifaseyin laarin ohun elo yii ati awọn ọlọjẹ miiran (HAV, HCV, DFV, HIV) fun wiwa nucleic acid pẹlu awọn ayẹwo ẹjẹ, ati awọn genomes eniyan. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR-gidi-gidi(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Eto Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Niyanju isediwon reagent: Makiro & Micro-Test Virus DNA / RNA Kit (HWTS-3017) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Test Aifọwọyi Nucleic Acid Extractor (HWTS-EQ011)) nipa Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Awọn isediwon yẹ ki o wa ni ti gbe jade ni ibamu si awọn 3 iwọn didun jade, 3 μL ti gbe jade ni ibamu si awọn 0. iwọn didun elution ti a ṣe iṣeduro jẹ 70μL.
