Ẹdọjẹdọ B Iwoye RNA
Orukọ ọja
HWTS-HP007 Hepatitis B Virus RNA Ohun elo Iwari Acid Acid (Fluorescence PCR)
Arun-arun
QWiwa ailagbara ti awọn ipele HBV-RNA ninu omi ara le ṣe atẹle dara julọ ipele ti HBV cccDNA ninu hepatocytes, ati ni akoko kanna yago fun ibajẹ nla si awọn alaisan.O jẹ iwulo nla fun ayẹwo iranlọwọ iranlọwọ ti ọlọjẹ jedojedo B (HBV) ati ibojuwo ipa ati asọtẹlẹ yiyọkuro oogun ti NAs itọju antiviral ni awọn alaisan CHB.
ikanni
| FAM | HBV-RNA |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | omi ara |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 100 idaako/ml |
| Ni pato | Awọn abajade idanwo kikọlu fihan pe ko si awọn ipa lori ipinnu HBV RNA nigbati ifọkansi ti bilirubin ninu omi ara ko ju 168.2μmol/mL, ifọkansi ti haemoglobin ti iṣelọpọ nipasẹ hemolysis ko ju 130g/L, ati pe ifọkansi ti awọn lipids ẹjẹ ko ju 65 mmol / milimita lọ;Ko si awọn ipa lori ipinnu HBV RNA nigbati apapọ ifọkansi IgG ninu omi ara ko ju 5mg/mL lọ.Awọn abajade idanwo ifaseyin agbekọja fihan pe ko si awọn aati agbelebu laarin ohun elo yii ati awọn ọlọjẹ miiran tabi awọn kokoro arun ti a rii nipasẹ acid nucleic ninu awọn ayẹwo ẹjẹ (ọlọjẹ ẹdọ C, cytomegalovirus eniyan, ọlọjẹ epstein-barr, ọlọjẹ ajẹsara eniyan, ọlọjẹ jedojedo A, syphilis , virus Herpes eniyan iru 6, Herpes simplex virus type 1, Herpes simplex virus type 2, influenza A virus, propionibacterium acnes, staphylococcus aureus, candida albicans) ati awọn genomes eniyan;lẹhin ti awọn alaisan ti mu lamivudine, telbivudine, adefovir dipivoxil ati entecavir, nigbati ẹru gbogun ti jedojedo B ninu ara wa loke LoD ti o kere ju ti ohun elo naa, ohun elo naa tun le rii daradara. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR SystemApplied Biosystems 7500 Yara Real-Time PCR Systems SLAN-96P Awọn ọna PCR gidi-akoko (Hongshi Medical Technology Co., Ltd.) |
Sisan iṣẹ
Aṣayan 1.
Viral DNA/RNA Isolation Kit (NA007-2) nipasẹ GenMag Biotechnology Co., Ltd. Awọn igbesẹ isediwon kan pato jọwọ tẹle ilana itọnisọna ni muna.
Makiro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Igbeyewo Aifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Awọn isediwon yẹ ki o wa ni ti gbe jade ni ibamu si awọn ilana itọnisọna, awọn jade iwọn didun ayẹwo jẹ 200μL, ati awọn niyanju elution iwọn didun ni 80μL.
