Iwoye Ẹdọgba E
Orukọ ọja
HWTS-HP006 Hepatitis E Iwoye Ohun elo Iwari Acid Acid (Fluorescence PCR)
Arun-arun
Kokoro Hepatitis E (HEV) jẹ ọlọjẹ RNA ti o fa awọn iṣoro ilera agbaye. O ni ibiti o ti gbalejo jakejado ati pe o ni ohun-ini ti awọn idena interspecies lila. O jẹ ọkan ninu awọn pathogens zoonotic pataki julọ ati fa ipalara nla si eniyan ati ẹranko. HEV ti wa ni gbigbe ni akọkọ nipasẹ gbigbe fecal-oral, ati pe o tun le tan kaakiri ni inaro nipasẹ awọn oyun tabi ẹjẹ. Lara wọn, ni ipa ọna gbigbe fecal-oral, omi ti a ti doti ti HEV ati ounjẹ tan kaakiri, ati ewu ikolu HEV ninu eniyan ati ẹranko jẹ giga[1-2].
ikanni
| FAM | HEV nucleic acid |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Ọfun swab |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 idaako / μL |
| Ni pato | Kokoro Hepatitis E (HEV) jẹ ọlọjẹ RNA ti o fa awọn iṣoro ilera agbaye. O ni ibiti o ti gbalejo jakejado ati pe o ni ohun-ini ti awọn idena interspecies lila. O jẹ ọkan ninu awọn pathogens zoonotic pataki julọ ati fa ipalara nla si eniyan ati ẹranko. HEV ti wa ni gbigbe ni akọkọ nipasẹ gbigbe fecal-oral, ati pe o tun le tan kaakiri ni inaro nipasẹ awọn oyun tabi ẹjẹ. Lara wọn, ni ipa ọna gbigbe fecal-oral, omi ti a ti doti ti HEV ati ounjẹ tan kaakiri, ati ewu ikolu HEV ninu eniyan ati ẹranko jẹ giga[1-2]. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR System QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR-gidi-gidi(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Eto Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1
Makiro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ati Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006). O yẹ ki o fa jade ni ibamu si awọn ilana. Iwọn didun ti a ṣe iṣeduro jẹ 80µL.
Aṣayan 2
TIANamp Virus DNA/RNA Kit (YDP315-R) ti a ṣelọpọ nipasẹ Tiangen Biotech (Beijing) Co., Ltd. O yẹ ki o fa jade ni ibamu si awọn ilana. Iwọn ayẹwo ti o jade jẹ 140μL. Iwọn igbejade ti a ṣeduro jẹ 60µL.v
