Cytomegalovirus eniyan (HCMV) Acid Nucleic
Orukọ ọja
HWTS-UR008A-ẹda eniyan cytomegalovirus (HCMV) ohun elo wiwa acid nucleic (Fluorescence PCR)
Arun-arun
cytomegalovirus eniyan (HCMV) jẹ ọmọ ẹgbẹ kan ti o ni jiini ti o tobi julọ ninu idile ọlọjẹ Herpes ati pe o le fi koodu sii ju awọn ọlọjẹ 200 lọ.HCMV ti wa ni ihamọ ni ihamọ ni ibiti ogun rẹ si eniyan, ati pe ko si awoṣe ẹranko ti ikolu rẹ.HCMV ni ọna ti o lọra ati gigun lati ṣe agbekalẹ ara ifisi inu intranuclear, ati ki o ma nfa iṣelọpọ ti perinuclear ati awọn ara ifisi cytoplasmic ati wiwu sẹẹli (awọn sẹẹli omiran), nitorinaa orukọ naa.Ni ibamu si awọn heterogeneity ti awọn oniwe-genome ati phenotype, HCMV le ti wa ni pin si orisirisi awọn igara, laarin eyi ti o wa ni o wa diẹ ninu awọn antigenic iyatọ, eyi ti, sibẹsibẹ, ni o wa ti ko si isẹgun lami.
Ikolu HCMV jẹ ikolu eto-ara, eyiti o ni awọn ẹya ara ti o pọju, ti o ni awọn aami aisan ti o ni idiwọn ati ti o yatọ, o wa ni ipalọlọ, ati pe o le fa ki awọn alaisan diẹ ṣe agbekalẹ awọn egbo ara-ọpọlọpọ pẹlu retinitis, jedojedo, pneumonia, encephalitis, colitis, monocytosis, ati thrombocytopenic. purpura.Ikolu HCMV wọpọ pupọ ati pe o han lati tan kaakiri agbaye.O jẹ ibigbogbo pupọ ninu olugbe, pẹlu awọn oṣuwọn iṣẹlẹ ti 45-50% ati diẹ sii ju 90% ni awọn orilẹ-ede ti o dagbasoke ati idagbasoke, lẹsẹsẹ.HCMV le dubulẹ ninu ara fun igba pipẹ.Ni kete ti ajesara ara ba ti di alailagbara, ọlọjẹ naa yoo mu ṣiṣẹ lati fa awọn aarun, paapaa awọn akoran ti nwaye ni awọn alaisan aisan lukimia ati awọn alaisan gbigbe, ati pe o le fa negirosisi ti ara ti ara ati ki o ṣe ewu igbesi aye awọn alaisan ni awọn ọran ti o lewu.Ni afikun si ibi iku, oyun ati ifijiṣẹ ti o ti tọjọ nipasẹ ikolu intrauterine, cytomegalovirus tun le fa awọn aiṣedeede ti aiṣedeede, nitorina ikolu HCMV ni anfani lati ni ipa lori prenatal ati abojuto postnatal ati didara olugbe.
ikanni
FAM | DNA HCMV |
VIC(HEX) | Iṣakoso ti abẹnu |
Imọ paramita
Ibi ipamọ | Omi: ≤-18℃ Ninu okunkun |
Selifu-aye | 12 osu |
Apeere Iru | Ayẹwo Serum, Ayẹwo Plasma |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 idaako / lenu |
Ni pato | Ko si ifaseyin agbekọja pẹlu ọlọjẹ jedojedo B, ọlọjẹ jedojedo C, ọlọjẹ papilloma eniyan, ọlọjẹ Herpes simplex type 1, virus herpes simplex type 2, awọn ayẹwo omi ara eniyan deede, ati bẹbẹ lọ. |
Awọn irinṣẹ to wulo: | O le baramu awọn ohun elo PCR Fuluorisenti akọkọ lori ọja naa. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-igbeyewo Aifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Isediwon yẹ ki o fa jade ni ibamu si awọn ilana naa.Iwọn ayẹwo isediwon jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
Niyanju isediwon reagent: QIAamp DNA Mini Kit (51304), Nucleic Acid isediwon tabi ìwẹnumọ Reagent (YDP315) nipa Tiangen Biotech (Beijing) Co., Ltd.yẹ ki o fa jade ni ibamu si awọn ilana isediwon, ati iwọn didun isediwon ti a ṣe iṣeduro jẹ 200 μL ati iwọn didun elution ti a ṣe iṣeduro jẹ 100 μL.