Eniyan PML-RARA Fusion Gene Iyipada
Orukọ ọja
HWTS-TM017APML-RARA Fusion Gene Iyipada Apo Ohun elo (Pluorescence PCR)
Arun-arun
Lukimia promyelocytic nla (APL) jẹ oriṣi pataki ti aisan lukimia myeloid nla (AML). Nipa 95% ti awọn alaisan APL wa pẹlu iyipada cytogenetic pataki kan, eyun t (15;17) (q22;q21), eyiti o jẹ ki apilẹṣẹ PML lori chromosome 15 ati retinoic acid receptor α gene (RARA) lori chromosome 17 dapọ lati ṣe agbekalẹ jiini idapọ PML-RARA. Nitori awọn aaye fifọ oriṣiriṣi ti jiini PML, apilẹṣẹ idapọ PML-RARA le pin si iru gigun (Iru L), iru kukuru (Iru S) ati iru iyatọ (Iru V), ṣiṣe iṣiro fun isunmọ 55%, 40% ati 5% lẹsẹsẹ.
ikanni
| FAM | Jiini idapọ PML-RARA |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ Ninu okunkun |
| Selifu-aye | osu 9 |
| Apeere Iru | ọra inu egungun |
| CV | <5.0 |
| LoD | 1000 idaako/ml. |
| Ni pato | Ko si ifasilẹ-agbelebu pẹlu awọn Jiini idapọ miiran BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, ati awọn Jiini idapọ TEL-AML1 |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR gidi-akoko (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Eto Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Niyanju isediwon reagent: RNAprep Pure Ẹjẹ Total RNA isediwon Apo (DP433). Iyọkuro yẹ ki o ṣe ni ibamu si IFU.
