Human ROS1 Fusion Gene iyipada
Orukọ ọja
HWTS-TM009-Eniyan ROS1 Apo Iwari Iyipada Gene (Fluorescence PCR)
Iwe-ẹri
CE
Arun-arun
ROS1 jẹ transmembrane tyrosine kinase ti idile olugba insulin.Jiini idapọ ROS1 ti jẹrisi bi jiini awakọ akàn ẹdọfóró miiran ti kii ṣe kekere sẹẹli.Gẹgẹbi aṣoju ti iru-ẹgbẹ molikula alailẹgbẹ tuntun kan, iṣẹlẹ ti jiini idapọ ROS1 ni NSCLC Nipa 1% si 2% ROS1 ni akọkọ n ṣe atunto pupọ ninu awọn exons 32, 34, 35 ati 36. Lẹhin ti o ti dapọ pẹlu awọn Jiini bii CD74, EZR, SLC34A2, ati SDC4, yoo tẹsiwaju lati mu agbegbe ROS1 tyrosine kinase ṣiṣẹ.ROS1 kinase ti a mu ṣiṣẹ laiṣedeede le mu awọn ipa ọna ifihan si isalẹ bi RAS/MAPK/ERK, PI3K/Akt/mTOR, ati JAK3/STAT3, nitorinaa kopa ninu isunmọ, iyatọ ati metastasis ti awọn sẹẹli tumo, ati nfa akàn.Lara awọn iyipada idapọ ROS1, CD74-ROS1 ṣe iroyin fun nipa 42%, awọn iroyin EZR fun nipa 15%, SLC34A2 fun nipa 12%, ati awọn iroyin SDC4 fun nipa 7%.Awọn ẹkọ-ẹkọ ti fihan pe aaye ATP-binding ti agbegbe catalytic ti ROS1 kinase ati aaye ATP-binding ti ALK kinase ni isomọ ti o to 77%, nitorina ALK tyrosine kinase kekere inhibitor molecule crizotinib ati bẹbẹ lọ ni ipa imularada ti o han gbangba. ni itọju ti NSCLC pẹlu iyipada idapọ ti ROS1.Nitorinaa, wiwa ti awọn iyipada idapọ ROS1 jẹ ipilẹ ile ati ipilẹ fun didari lilo awọn oogun crizotinib.
ikanni
| FAM | Idaduro Idahun 1, 2, 3 ati 4 |
| VIC(HEX) | Idaduro Idahun 4 |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | paraffin ti a fi sinu iṣan pathological tabi awọn ayẹwo ti ge wẹwẹ |
| CV | 5.0% |
| Ct | ≤38 |
| LoD | Ohun elo yii le ṣe awari awọn iyipada idapọ bi kekere bi 20 ẹda. |
| Awọn irinṣẹ to wulo: | Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7500 Yara Real-Time PCR Systems SLAN ®-96P Real-Time PCR Systems QuantStudio™ 5 Awọn ọna PCR-gidi-gidi LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Niyanju isediwon reagent: RNeasy FFPE Kit (73504) lati QIAGEN, Paraffin ifibọ Tissue Apa Total RNA isediwon Apo (DP439) lati Tiangen Biotech (Beijing) Co., Ltd.
