Aarun ayọkẹlẹ A Iwoye Agbaye/H1/H3
Orukọ ọja
HWTS-RT012 Aarun ayọkẹlẹ A Iwoye Agbaye/H1/H3 Apo Iwari Oniruuru Acid (Fluorescence PCR)
Arun-arun
Kokoro aarun ayọkẹlẹ jẹ ẹya aṣoju ti Orthomyxoviridae. O jẹ pathogen ti o ṣe ewu ilera eniyan ni pataki. O le ṣe akoran agbalejo lọpọlọpọ. Ajakale akoko naa kan nipa 600 milionu eniyan ni agbaye ati pe o fa iku 250,000 ~ 500,000, eyiti kokoro aarun ayọkẹlẹ A jẹ idi akọkọ ti ikolu ati iku. Kokoro aarun ayọkẹlẹ jẹ RNA ti o ni odi-okun-okun kan. Ni ibamu si awọn dada hemagglutinin (HA) ati neuraminidase (NA), HA le ti wa ni pin si 16 subtypes, NA Pin si 9 subtypes. Lara awọn ọlọjẹ aarun ayọkẹlẹ A, awọn oriṣi ti awọn ọlọjẹ aarun ayọkẹlẹ ti o le ṣe akoran taara eniyan ni: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 ati H10N8. Lara wọn, H1 ati H3 subtypes jẹ pathogenic pupọ, ati pe o yẹ akiyesi pataki.
ikanni
| FAM | aarun ayọkẹlẹ A gbogbo iru kokoro nucleic acid |
| VIC/HEX | aarun ayọkẹlẹ A H1 iru kokoro nucleic acid |
| ROX | aarun ayọkẹlẹ A H3 iru kokoro nucleic acid |
| CY5 | ti abẹnu Iṣakoso |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 idaako / μL |
| Ni pato | Ko si ifaseyin agbelebu pẹlu awọn ayẹwo atẹgun miiran gẹgẹbi Aarun ayọkẹlẹ A, Aarun ayọkẹlẹ B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Virus Syncytial Respiratory, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneu2 virus, Metapneu2. ọlọjẹ syncytial A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, ati bẹbẹ lọ ati DNA genomic eniyan. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR-gidi-gidi(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Eto Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Iyọkuro Acid Nucleic tabi Reagent Iwẹnumọ (YDP315-R) nipasẹ Tiangen Biotech (Beijing) Co., Ltd. Awọn isediwon yẹ ki o wa ni ti gbe jade muna ni ibamu si awọn ilana fun lilo. Iwọn ayẹwo ti o jade jẹ 140μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 60μL.
Aṣayan 2.
Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Awọn isediwon yẹ ki o wa ni ti gbe jade muna ni ibamu si awọn ilana fun lilo. Iwọn ayẹwo ti o jade jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
