Aarun ayọkẹlẹ A Iwoye Agbaye/H1/H3
Orukọ ọja
HWTS-RT012 Aarun ayọkẹlẹ A Iwoye Agbaye/H1/H3 Apo Iwari Oniruuru Acid (Fluorescence PCR)
Arun-arun
Mycoplasma pneumoniae (MP) jẹ microorganism prokaryotic ti o kere julọ pẹlu eto sẹẹli ati pe ko si odi sẹẹli laarin awọn kokoro arun ati awọn ọlọjẹ.MP ni akọkọ fa awọn akoran atẹgun atẹgun ninu eniyan, paapaa ni awọn ọmọde ati awọn ọdọ.MP le fa Mycoplasma hominis pneumonia, awọn akoran atẹgun atẹgun ninu awọn ọmọde ati pneumonia atypical.Awọn aami aisan ile-iwosan yatọ, pupọ julọ Ikọaláìdúró, iba, otutu, orififo, ọfun ọfun, ikolu ti atẹgun atẹgun oke ati bronchopneumonia ni o wọpọ julọ.Diẹ ninu awọn alaisan le ni idagbasoke pneumonia ti o lagbara lati ikolu ti atẹgun atẹgun oke, ati ipọnju atẹgun ti o lagbara tabi iku paapaa le waye.MP jẹ ọkan ninu awọn pathogens ti o wọpọ ati pataki ni pneumonia ti agbegbe (CAP), ti o ṣe iṣiro 10% -30% ti CAP, ati pe ipin le pọ si awọn akoko 3-5 nigbati MP ba wa ni ibigbogbo.Ni awọn ọdun aipẹ, ipin ti MP ninu awọn pathogens CAP ti pọ si diẹdiẹ.Iṣẹlẹ ti Mycoplasma pneumoniae ikolu ti pọ si, ati nitori awọn ifihan ile-iwosan ti ko ni pato, o rọrun lati dapo pelu kokoro-arun ati awọn otutu ọlọjẹ.Nitorinaa, wiwa ile-iwosan ni kutukutu jẹ pataki nla fun ayẹwo ile-iwosan ati itọju.
ikanni
| FAM | aarun ayọkẹlẹ A gbogbo iru kokoro nucleic acid |
| VIC/HEX | aarun ayọkẹlẹ A H1 iru kokoro nucleic acid |
| ROX | aarun ayọkẹlẹ A H3 iru kokoro nucleic acid |
| CY5 | ti abẹnu Iṣakoso |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 idaako / μL |
| Ni pato | Ko si ifaseyin agbelebu pẹlu awọn ayẹwo atẹgun miiran gẹgẹbi Aarun ayọkẹlẹ A, Aarun ayọkẹlẹ B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Virus Syncytial Respiratory, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneu2 virus B1/B2, Kokoro syncytial ti atẹgun A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, bbl ati DNA genomic eniyan. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR-gidi-gidi(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Eto Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Iyọkuro Acid Nucleic tabi Reagent Iwẹnumọ (YDP315-R) nipasẹ Tiangen Biotech (Beijing) Co., Ltd.Awọn isediwon yẹ ki o wa ni ti gbe jade muna ni ibamu si awọn ilana fun lilo.Iwọn ayẹwo ti o jade jẹ 140μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 60μL.
Aṣayan 2.
Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Awọn isediwon yẹ ki o wa ni ti gbe jade muna ni ibamu si awọn ilana fun lilo.Iwọn ayẹwo ti o jade jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
