Klebsiella Pneumoniae, Acinetobacter Baumannii ati Pseudomonas Aeruginosa ati Awọn Jiini Resistance Drug (KPC, NDM, OXA48 ati IMP) Multiplex
Orukọ ọja
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii ati Pseudomonas Aeruginosa ati Awọn Jiini Resistance Drug (KPC, NDM, OXA48 ati IMP) Apo Iwari Multiplex (Fluorescence PCR)
Iwe-ẹri
CE
Arun-arun
Klebsiella pneumoniae jẹ pathogen opportunistic ile-iwosan ti o wọpọ ati ọkan ninu awọn kokoro arun pathogenic pataki ti o nfa awọn akoran ile-iṣẹ.Nigba ti agbara ara ba dinku, awọn kokoro arun wọ inu ẹdọforo lati inu atẹgun atẹgun, ti o fa ikolu ni awọn ẹya pupọ ti ara, ati lilo awọn egboogi ni kutukutu jẹ bọtini lati ṣe iwosan.[1].
Aaye ti o wọpọ julọ ti ikolu Acinetobacter baumannii jẹ ẹdọforo, eyiti o jẹ pathogen pataki fun pneumonia ti o gba (HAP), paapaa Ventilator ti o ni ibatan pneumonia (VAP).Nigbagbogbo o tẹle pẹlu awọn akoran kokoro-arun miiran ati olu, pẹlu awọn abuda ti oṣuwọn aarun giga ati oṣuwọn iku giga.
Pseudomonas aeruginosa jẹ bacilli ti kii-fermentative gram-negative ti o wọpọ julọ ni adaṣe ile-iwosan, ati pe o jẹ pathogen opportunistic pataki fun ikolu ti ile-iwosan ti o gba, pẹlu awọn abuda ti imunisin ti o rọrun, iyatọ ti o rọrun ati ilodisi olona-oògùn.
ikanni
| Oruko | PCR-Idapọ 1 | PCR-Idapọ 2 |
| FAM ikanni | Aba | IMP |
| VIC / HEX ikanni | Iṣakoso ti abẹnu | KPC |
| CY5 ikanni | PA | NDM |
| ROX ikanni | KPN | OXA48 |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Sputum |
| Ct | ≤36 |
| CV | ≤10.0% |
| LoD | 1000 CFU/ml |
| Ni pato | A) Idanwo ifasilẹ-agbelebu fihan pe ohun elo yii ko ni ifaseyin agbelebu pẹlu awọn aarun atẹgun miiran, gẹgẹ bi Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter, hemolyticamonylosis, Acinetobacter hemolytic, Pneumoniae fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus ati sputum samples laisi awọn ibi-afẹde, ati bẹbẹ lọ. b) Agbara kikọlu: Yan mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, ati roxithromycin, ati bẹbẹ lọ fun idanwo kikọlu, ati awọn abajade fihan pe awọn nkan kikọlu ti a mẹnuba loke. maṣe dabaru pẹlu wiwa Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa ati awọn jiini resistance carbapenem KPC, NDM, OXA48 ati IMP. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Eto Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Lapapọ PCR Solusan
