Kokoro Monkeypox ati Titẹ Nucleic Acid

Apejuwe kukuru:

Ohun elo yii ni a lo fun wiwa agbara in vitro ti ọlọjẹ monkeypox clade I, clade II ati ọlọjẹ monkeypox gbogbo nucleic acids ninu omi sisu eniyan, swabs oropharyngeal ati awọn ayẹwo omi ara.

Fi imeeli ranṣẹ si wa

Alaye ọja

ọja Tags

Orukọ ọja

HWTS-OT202-Iwoye Abọbọ ati Titẹ Apo Iwari Acid Nucleic (Pluorescence PCR)

Iwe-ẹri

Arun-arun

Monkeypox (Mpox) jẹ arun àkóràn zoonotic ńlá kan ti o fa nipasẹ Iwoye Abọbọ (MPXV). MPXV jẹ biriki yika tabi oval ni apẹrẹ, ati pe o jẹ ọlọjẹ DNA ti o ni ilopo meji pẹlu ipari ti bii 197Kb^[1]. Arun naa ni o maa n tan kaakiri nipasẹ awọn ẹranko, ati pe eniyan le ni akoran nipa jijẹ awọn ẹranko ti o ni arun jẹ tabi nipa ifarakan taara pẹlu ẹjẹ, awọn omi ara ati sisu ti awọn ẹranko ti o ni arun. Kokoro naa tun le tan kaakiri laarin awọn eniyan, nipataki nipasẹ awọn isunmi atẹgun lakoko gigun, olubasọrọ oju-si-oju taara tabi nipasẹ olubasọrọ taara pẹlu awọn omi ara alaisan tabi awọn nkan ti o doti.^[2-3]. Awọn ẹkọ-ẹkọ ti fihan pe MPXV ṣe awọn ipele meji ọtọtọ: clade I (eyiti a mọ tẹlẹ bi Central African clade tabi Congo Basin clade) ati clade II (ti a npe ni Iwo-oorun Afirika tẹlẹ). Mpox ti Congo Basin clade ti han gbangba pe o jẹ gbigbe laarin awọn eniyan ati pe o le fa iku, lakoko ti o ti jẹ ki o fa awọn aami aiṣan ti o kere ju ati pe o ni iwọn kekere ti gbigbe eniyan-si-eniyan.^[4].

Awọn abajade idanwo ti ohun elo yii ko ni ipinnu lati jẹ itọkasi nikan fun ayẹwo ti akoran MPXV ninu awọn alaisan, eyiti o gbọdọ ni idapo pẹlu awọn abuda ile-iwosan ti alaisan ati data idanwo yàrá miiran lati ṣe idajọ ikolu ti pathogen ni deede ati ṣe agbekalẹ eto itọju ti o tọ lati rii daju ailewu ati itọju to munadoko.

ikanni

FAM	MPXV clade II
ROX	MPXV acid nucleic agbaye
VIC/HEX	MPXV clade I
CY5	ti abẹnu Iṣakoso

Imọ paramita

Ibi ipamọ	≤-18℃
Selifu-aye	12 osu
Apeere Iru	omi sisu eniyan, oropharyngeal swabs ati omi ara
Ct	≤38 (FAM, VIC/HEX, ROX), ≤35（IC)
LoD	200 idaako/ml
Awọn ohun elo ti o wulo	Iru I ri reagenti: Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio^®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR-gidi-gidi (Ẹrọ imọ-ẹrọ Iṣoogun Hongshi Co., Ltd.) LightCycler^®480 Real-Time PCR eto LineGene 9600 Plus Awọn ọna Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System Iru II reagent iwari: Eudemon^TMAIO800 (HWTS-EQ007)) nipasẹ Jiangsu Makiro & Micro-Test Med-Tech Co., Ltd.