MTHFR Gene Polymorphic Acid Nucleic
Orukọ ọja
HWTS-GE004-MTHFR Apo Iwari Acid Nucleic Acid Gene Polymorphic (ARMS-PCR)
Arun-arun
Folic acid jẹ Vitamin ti o le ni omi ti o jẹ cofactor pataki ninu awọn ipa ọna iṣelọpọ ti ara. Ni awọn ọdun aipẹ, nọmba nla ti awọn ijinlẹ ti jẹrisi pe, iyipada ti folate metabolizing henensiamu gene MTHFR yoo ja si aipe folic acid ninu ara, ati ibajẹ ti o wọpọ ti aipe folic acid ninu awọn agbalagba le fa ẹjẹ megaloblastic, ibajẹ endothelial ti iṣan, bbl Folic acid aipe ninu awọn aboyun ko le pade awọn aini ti ara wọn ati awọn oyun inu oyun, ati oyun. Awọn ipele folate omi ara ni ipa nipasẹ 5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms. Awọn iyipada 677C>T ati 1298A>C ninu jiini MTHFR fa iyipada ti alanine si valine ati glutamic acid, ni atẹlera, ti o fa idinku iṣẹ MTHFR dinku ati nitorinaa dinku lilo folic acid.
ikanni
| FAM | MTHFR C677T |
| ROX | MTHFR A1298C |
| VIC(HEX) | Iṣakoso inu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Ẹjẹ anticoagulated EDTA tuntun ti a gba |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Awọn irinṣẹ to wulo: | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems SLAN ®-96P Real-Time PCR Systems QuantStudio™ 5 Awọn ọna PCR-gidi-gidi LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1
Niyanju isediwon reagents: Makiro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) ati Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Aṣayan 2
Niyanju isediwon reagents: Ẹjẹ Genomic DNA isediwon Apo (YDP348, JCXB20210062) nipa Tiangen Biotech(Beijing) Co., Ltd. Apo Iyọkuro Jiini Ẹjẹ (A1120) nipasẹ Promega.
