Mumps Iwoye Nucleic Acid
Orukọ ọja
HWTS-RT029-Iwoye Iwoye Acid Nucleic Acid (Pluorescence PCR)
Arun-arun
Kokoro mumps jẹ ọlọjẹ serotype kan, ṣugbọn jiini amuaradagba SH jẹ iyipada pupọ ni awọn ọlọjẹ mumps oriṣiriṣi. Kokoro mumps ti pin si awọn genotypes 12 ti o da lori awọn iyatọ ti awọn jiini amuaradagba SH, eyun awọn iru A, B, C, D, F, G, H, I, J, K, L, ati N. Pipin awọn genotypes kokoro mumps ni awọn abuda agbegbe ti o han gbangba. Awọn igara ti o gbilẹ ni Yuroopu jẹ nipataki awọn genotypes A, C, D, G, ati H; Awọn igara ti o wọpọ ni Amẹrika jẹ awọn genotypes C, D, G, H, J, ati K; Awọn igara ti o wọpọ ni Asia jẹ awọn genotypes B, F, I, ati L; Iwọn akọkọ ti o wọpọ ni Ilu China jẹ genotype F; awọn igara ti o wọpọ ni Japan ati South Korea jẹ genotypes B ati Emi ni atele. Ko ṣe akiyesi boya titẹ ọlọjẹ ti o da lori jiini SH jẹ itumọ fun iwadii ajesara. Lọwọlọwọ, Awọn igara ajesara laaye laaye ni lilo ni agbaye jẹ genotype A ni pataki, ati awọn ọlọjẹ ti a ṣe nipasẹ awọn antigens ọlọjẹ ti awọn oriṣiriṣi genotypes jẹ aabo agbelebu.
Imọ paramita
| Ibi ipamọ | -18 ℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | Ọfun swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 1000 idaako/ml |
| Awọn ohun elo ti o wulo | O wulo lati tẹ reagent iwari I: Ohun elo Biosystems 7500 Real-akoko PCR Systems, QuantStudio®5 Awọn ọna PCR akoko gidi, SLAN-96P Awọn ọna PCR Akoko-gidi (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Awọn ọna Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer), MA-6000 Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Eto PCR akoko-gidi BioRad CFX96, BioRad CFX Opus 96 Real-Time PCR System. Wulo fun iru II reagent iwari: EudemonTMAIO800 (HWTS-EQ007) nipasẹ Jiangsu Makiro & Micro-Test Med-Tech Co., Ltd. |
Sisan iṣẹ
Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (eyiti o le ṣee lo pẹlu Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), ati Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8 le ṣee lo)TM AIO800 (HWTS-EQ007)) nipasẹ Jiangsu Makiro & Micro-Test Med-Tech Co., Ltd.
Iwọn ayẹwo ti o jade jẹ 200μL ati iwọn didun elution ti a ṣe iṣeduro jẹ 150μL.
