Mycobacterium Tuberculosis Nucleic Acid ati Rifampicin (RIF) ， Isoniazid Resistance (INH)

Mycobacterium Tuberculosis Acid Nucleic Acid ati Rifampicin(RIF) ， Isoniazid Resistance(INH) Aworan Ifihan

Apejuwe kukuru:

Ọja yii dara fun wiwa didara ti Mycobacterium iko DNA ninu awọn ayẹwo sputum eniyan ni vitro, bakanna bi iyipada homozygous ni agbegbe 507-533 amino acid codon (81bp, agbegbe ti npinnu resistance rifampicin) ti jiini rpoB ti o fa ikọ-ara Mycobacterium rifampicin resistance.

Fi imeeli ranṣẹ si wa

Alaye ọja

ọja Tags

Orukọ ọja

HWTS-RT147 Mycobacterium Tuberculosis Acid Nucleic Acid ati Rifampicin(RIF), Ohun elo Iwari Isoniazid (INH)

Arun-arun

Ikọ-ẹ̀gbẹ Mycobacterium, laipẹ bi Tubercle bacillus (TB), jẹ kokoro arun alamọja ti o fa iko.Lọwọlọwọ, awọn oogun egboogi-egboogi-ila akọkọ ti a nlo nigbagbogbo pẹlu isoniazid, rifampicin ati ethambutol, ati bẹbẹ lọ. Sibẹsibẹ, nitori lilo ti ko tọ ti awọn oogun egboogi-ikọ-ara ati awọn abuda ti eto ogiri sẹẹli ti iko-ara mycobacterium, iko-ara mycobacterium ndagba resistance oogun si awọn oogun ikọ-ara, eyiti o mu awọn italaya pataki si idena ati itọju ikọ-igbẹ.

ikanni

Orukọ afojusun			Onirohin	Quencher
Idaduro lenuA	Idaduro lenuB	Idaduro lenuC	Onirohin	Quencher
rpoB 507-514	rpoB 513-520	IS6110	FAM	Ko si
rpoB 520-527	rpoB 527-533	/	CY5	Ko si
/	/	Iṣakoso inu	HEX(VIC)	Ko si

Idaduro lenuD	Onirohin	Quencher
Agbegbe olupolowo InhA -15C>T, -8T>A, -8T>C	FAM	Ko si
KatG 315 codon 315G>A,315G>C	CY5	Ko si
AhpC agbegbe olupolowo -12C>T, -6G>A	ROX	Ko si

Imọ paramita

Ibi ipamọ	≤-18℃
Selifu-aye	12 osu
Apeere Iru	sputum
CV	≤5.0%
LoD	LoD ti mycobacterium tuberculosis ti orilẹ-ede itọkasi jẹ 50 kokoro arun/mL.LoD ti iru egan-sooro rifampicin ni itọkasi orilẹ-ede jẹ 2×10³kokoro arun / milimita, ati LoD ti iru mutant jẹ 2×10³kokoro arun/ml.LoD ti awọn kokoro arun isoniazid sooro iru egan jẹ 2x10³kokoro arun / milimita, ati LoD ti kokoro arun mutant jẹ 2x10³kokoro arun/ml.
Ni pato	Awọn abajade idanwo agbelebu fihan pe ko si ifaseyin agbelebu ni wiwa ti jiini eniyan, awọn mycobacteria miiran ti ko ni iko ati awọn ọlọjẹ pneumonia pẹlu ohun elo yii;Ko si esi agbelebu ti a rii ni awọn aaye iyipada ti awọn jiini sooro oogun miiran ninu iko-ara Mycobacterium ti igbẹ.
Awọn ohun elo ti o wulo	SLAN-96P Awọn ọna PCR-gidi-gidi (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Eto PCR akoko-gidi, Imọ-ẹrọ Hangzhou Bioer QuantGene 9600 Eto PCR gidi-akoko, QuantStudio^®5 Real-Time PCR System.