Mycobacterium Tuberculosis Nucleic Acid ati Rifampicin Resistance
Orukọ ọja
HWTS-RT074B-Mycobacterium Tuberculosis Acid Nucleic Acid ati Rifampicin Resistance Apo (Ibi Irọ)
Iwe-ẹri
CE
Arun-arun
Mycobacterium iko , Kó bi Tubercle bacillus, jẹdọjẹdọ, ni pathogenic kokoro arun ti o fa iko.Lọwọlọwọ, awọn oogun egboogi-egboogi-ila akọkọ ti a nlo nigbagbogbo pẹlu isoniazid, rifampicin ati hexambutol, ati bẹbẹ lọ. Sibẹsibẹ, nitori lilo ti ko tọ ti awọn oogun egboogi-ikọ-ara ati awọn abuda ti eto ogiri sẹẹli ti iko-ara mycobacterium, iko-ara mycobacterium ndagba resistance oogun si awọn oogun ikọ-ara, eyiti o mu awọn italaya pataki si idena ati itọju ikọ-igbẹ.
Rifampicin ti jẹ lilo pupọ ni itọju awọn alaisan iko ẹdọforo lati opin awọn ọdun 1970, ati pe o ni ipa pataki.O ti jẹ yiyan akọkọ lati kuru chemotherapy ti awọn alaisan iko ẹdọforo.Idaduro Rifampicin jẹ pataki nipasẹ iyipada ti jiini rpoB.Botilẹjẹpe awọn oogun egboogi-ikọ-ara tuntun n jade nigbagbogbo, ati pe ipa ile-iwosan ti awọn alaisan iko ẹdọforo tun ti tẹsiwaju lati ni ilọsiwaju, aini ibatan tun wa ti awọn oogun egboogi-ikọ-ara, ati lasan ti lilo oogun aiṣedeede ni ile-iwosan ga ni iwọn.Ó ṣe kedere pé ikọ́ ẹ̀gbẹ Mycobacterium nínú àwọn aláìsàn tí wọ́n ní ikọ́ ẹ̀dọ̀fóró kò lè pa á pátápátá ní àkókò tó yẹ, èyí tó máa ń yọrí sí oríṣiríṣi ìwọ̀n ìgbógunti oògùn nínú ara aláìsàn, ó máa ń fa àrùn náà gùn, ó sì máa ń mú kí ewu ikú aláìsàn pọ̀ sí i.
ikanni
| ikanni | Awọn ikanni ati Fluorophores | Idaduro Idahun A | Idaduro Idahun B | Idaduro Idahun C |
| FAM ikanni | Onirohin: FAM, Quencher: Ko si | rpoB 507-514 | rpoB 513-520 | 38KD ati IS6110 |
| CY5 ikanni | Onirohin: CY5, Quencher: Kò | rpoB 520-527 | rpoB 527-533 | / |
| HEX (VIC) ikanni | Onirohin: HEX (VIC), Quencher: Kò | Iṣakoso inu | Iṣakoso inu | Iṣakoso inu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ Ninu okunkun |
| Selifu-aye | 12 osu |
| Apeere Iru | Sputum |
| CV | ≤5.0% |
| LoD | iko mycobacterium 50 kokoro arun / milimita rifampicin-sooro egan iru: 2x103kokoro arun/ml eda eniyan fohun: 2x103kokoro arun/ml |
| Ni pato | O ṣe awari iko-ara mycobacterium-iru-igbẹ ati awọn aaye iyipada ti awọn jiini resistance oogun miiran gẹgẹbi katG 315G>C\A, InhA-15C> T, awọn abajade idanwo ko fihan atako si rifampicin, eyiti o tumọ si pe ko si ifaseyin agbelebu. |
| Awọn irinṣẹ to wulo: | SLAN-96P Real-Time PCR Systems BioRad CFX96 Real-Time PCR System LightCycler480® Real-Time PCR System |
Sisan iṣẹ
Ti o ba lo Makiro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (eyiti o le ṣee lo pẹlu Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) tabi Makiro & Micro-Test Viral DNA/RNA Column (HWTS-3022-50) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. fun isediwon, fi kun. 200μL ti Iṣakoso Rere, iṣakoso odi ati ayẹwo sputum ti a ti ni ilọsiwaju lati ṣe idanwo ni ọkọọkan, ati ṣafikun 10μL ti iṣakoso inu lọtọ sinu Iṣakoso Rere, iṣakoso odi ati apẹẹrẹ sputum ti a ṣe ilana lati ṣe idanwo, ati awọn igbesẹ atẹle yẹ ki o wa ni muna. ni ibamu si awọn ilana isediwon.Iwọn ayẹwo ti o jade jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 100μL.
