Mycobacterium Tuberculosis Nucleic Acid ati Rifampicin Resistance
Orukọ ọja
HWTS-RT074B-Mycobacterium Tuberculosis Acid Nucleic Acid ati Rifampicin Resistance Apo (Ibi Irọ)
Iwe-ẹri
CE
Arun-arun
Mycobacterium iko , Kó bi Tubercle bacillus, jẹdọjẹdọ, ni pathogenic kokoro arun ti o fa iko. Lọwọlọwọ, awọn oogun egboogi-egboogi-ila akọkọ ti a lo nigbagbogbo pẹlu isoniazid, rifampicin ati hexambutol, ati bẹbẹ lọ. iko, mycobacterium iko ndagba oògùn resistance si egboogi-ikolu, eyi ti o mu pataki italaya si idena ati itoju ti iko.
Rifampicin ti jẹ lilo pupọ ni itọju awọn alaisan iko ẹdọforo lati opin awọn ọdun 1970, ati pe o ni ipa pataki. O ti jẹ yiyan akọkọ lati kuru chemotherapy ti awọn alaisan iko ẹdọforo. Idaduro Rifampicin jẹ pataki nipasẹ iyipada ti jiini rpoB. Botilẹjẹpe awọn oogun egboogi-ikọ-ara tuntun n jade nigbagbogbo, ati pe ipa ile-iwosan ti awọn alaisan iko ẹdọforo tun ti tẹsiwaju lati ni ilọsiwaju, aini ibatan kan tun wa ti awọn oogun egboogi-ikọ-ara, ati iṣẹlẹ ti lilo oogun aiṣedeede ni ile-iwosan ga ni iwọn. Ó ṣe kedere pé ikọ́ ẹ̀gbẹ Mycobacterium tí ó wà nínú àwọn aláìsàn tí wọ́n ní ikọ́ ẹ̀dọ̀fóró kò lè pa wọ́n pátápátá ní ọ̀nà tí ó tọ́, èyí tí ó máa ń yọrí sí oríṣiríṣi ìwọ̀n ìgbógunti oògùn nínú ara aláìsàn, yóò mú kí àrùn náà gùn, ó sì ń mú kí ewu ikú aláìsàn pọ̀ sí i.
ikanni
| ikanni | Awọn ikanni ati Fluorophores | Idaduro Idahun A | Idaduro Idahun B | Idaduro Idahun C |
| FAM ikanni | Onirohin: FAM, Quencher: Ko si | rpoB 507-514 | rpoB 513-520 | 38KD ati IS6110 |
| CY5 ikanni | Onirohin: CY5, Quencher: Kò | rpoB 520-527 | rpoB 527-533 | / |
| HEX (VIC) ikanni | Onirohin: HEX (VIC), Quencher: Ko si | Iṣakoso inu | Iṣakoso inu | Iṣakoso inu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ Ninu okunkun |
| Selifu-aye | 12 osu |
| Apeere Iru | Tutu |
| CV | ≤5.0% |
| LoD | iko mycobacterium 50 kokoro arun / milimita rifampicin-sooro egan iru: 2x103kokoro arun/ml eda eniyan fohun: 2x103kokoro arun/ml |
| Ni pato | O ṣe awari iko-ara mycobacterium iru-igbẹ ati awọn aaye iyipada ti awọn jiini resistance oogun miiran gẹgẹbi katG 315G>C\A, InhA-15C> T, awọn abajade idanwo ko fihan pe ko si resistance si rifampicin, eyiti o tumọ si pe ko si ifaseyin agbelebu. |
| Awọn irinṣẹ to wulo: | SLAN-96P Real-Time PCR Systems BioRad CFX96 Real-Time PCR System LightCycler480® Real-Time PCR System |
Sisan iṣẹ
Ti o ba lo Makiro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Test Aifọwọyi Nucleic Acid Extractor (HWTS-3019-30) Macro. Micro-Test Viral DNA / RNA Column (HWTS-3022-50) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. fun isediwon, ṣafikun 200μL ti Iṣakoso Rere, iṣakoso odi ati apẹẹrẹ sputum ti a ṣe ilana lati ṣe idanwo ni ọkọọkan, ati ṣafikun 10μL ti iṣakoso inu inu lọtọ, ati iṣakoso odi ni idanwo sputum lọtọ, ati iṣakoso odi ti iṣakoso ni lọtọ. awọn igbesẹ atẹle yẹ ki o wa ni muna ni ibamu si awọn ilana isediwon. Iwọn ayẹwo ti o jade jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 100μL.
