Mycoplasma Pneumoniae (MP)
Orukọ ọja
HWTS-RT024 Mycoplasma Pneumoniae (MP) Ohun elo Iwari Acid Nucleic (Fluorescence PCR)
Arun-arun
Mycoplasma pneumoniae (MP) jẹ iru microorganism prokaryotic ti o kere julọ, eyiti o wa laarin awọn kokoro arun ati ọlọjẹ, pẹlu eto sẹẹli ṣugbọn ko si odi sẹẹli. MP ni akọkọ fa ikolu ti atẹgun atẹgun eniyan, paapaa ni awọn ọmọde ati awọn ọdọ. O le fa eniyan mycoplasma pneumonia, ikolu ti atẹgun ti awọn ọmọde ati pneumonia atypical. Awọn ifarahan ile-iwosan jẹ oriṣiriṣi, pupọ julọ eyiti o jẹ Ikọaláìdúró nla, iba, otutu, orififo, ọfun ọfun. Ikolu apa atẹgun ti oke ati pneumonia bron jẹ eyiti o wọpọ julọ. Diẹ ninu awọn alaisan le ni idagbasoke lati ikolu ti atẹgun atẹgun oke si pneumonia ti o lagbara, ipọnju atẹgun ti o lagbara ati iku le waye.
ikanni
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Sputum, Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 idaako/ml |
| Ni pato | a) Agbelebu reactivity: ko si ifaseyin agbelebu pẹlu Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Klebsiella pneumoniae, Staphylococcus aureone, Staphylococcus aureone. pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, Aarun ayọkẹlẹ Aarun ayọkẹlẹ, Aarun ayọkẹlẹ B kokoro, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus and human genomic nucleic acid. Agbara kikọlu: ko si kikọlu nigbati a ṣe idanwo awọn nkan ti o ni idiwọ pẹlu awọn ifọkansi wọnyi: haemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) ẹjẹ eniyan, levofloxacin (10μg/mL), moxifloxacin (0μg/L), moxifloxacin (80μg/ml), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR gidi-akoko (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Eto Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
(1) Ayẹwo Sputum
Niyanju isediwon reagent: Makiro & Micro-igbeyewo Gbogun ti DNA/RNA Apo (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-West laifọwọyi Nucleic Acid Extractor (30H6) HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ṣafikun 200µL ti iyọ deede si ojoro ti a ṣe ilana. Iyọkuro atẹle yẹ ki o ṣe ni ibamu si itọnisọna fun lilo. Iwọn elution ti a ṣe iṣeduro jẹ 80µL. Iṣeduro isediwon ti a ṣe iṣeduro: Iyọkuro Acid Nucleic Acid tabi Reagent Mimọ (YDP315-R). Iyọkuro yẹ ki o ṣee ṣe ni ibamu si itọnisọna fun lilo. Iwọn didun ti a ṣe iṣeduro jẹ 60µL.
(2) Oropharyngeal swab
Niyanju isediwon reagent: Makiro & Micro-igbeyewo Gbogun ti DNA/RNA Apo (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-West laifọwọyi Nucleic Acid Extractor (30H6) HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Isediwon yẹ ki o ṣe ni ibamu si itọnisọna fun lilo. Iwọn isediwon ti a ṣe iṣeduro ti ayẹwo jẹ 200µL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80µL. Iṣeduro isediwon ti a ṣe iṣeduro: QIAamp Viral RNA Mini Kit (52904) tabi Iyọkuro Acid Nucleic tabi Reagent Isọdimilẹ (YDP315-R). Iyọkuro yẹ ki o ṣee ṣe ni ibamu si itọnisọna fun lilo. Iwọn isediwon ti a ṣeduro fun apẹẹrẹ jẹ 140µL, ati iwọn didun elution ti a ṣeduro jẹ 60µL.
