Clostridium difficile toxin A/B gene (C.diff)
Orukọ ọja
HWTS-OT031A Ohun elo Iwari Acid Nucleic fun Clostridium difficile toxin A/B gene (C.diff) (Pluorescence PCR)
Iwe-ẹri
CE
Arun-arun
Clostridium difficile (CD), gira-dara anaerobic sporogenic difficile Clostridium difficile, jẹ ọkan ninu awọn pathogens akọkọ ti o nfa awọn akoran ifun nosocomial.Ni ile-iwosan, nipa 15% ~ 25% ti gbuuru ti o ni ibatan antimicrobial, 50% ~ 75% ti colitis ti o ni ibatan antimicrobial ati 95% ~ 100% ti enteritis pseudomembranous jẹ eyiti o fa nipasẹ Clostridium difficile infection (CDI).Clostridium difficile jẹ pathogen ni àídájú, pẹlu awọn igara majele ati awọn igara ti kii ṣe majele.
ikanni
| FAM | tcdAàbùdá |
| ROX | tcdBàbùdá |
| VIC/HEX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | otita |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/ml |
| Ni pato | lo ohun elo yii lati ṣawari awọn aarun inu ifun miiran bi Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile ti kii-patogeniki igara, Adenovirus, rotavirus, norovirus, influenza A virus, influenza virus and human gefluenza virus. DNA, awọn abajade jẹ gbogbo odi. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR gidi-akoko (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Eto Wiwa PCR akoko-gidi(FQD-96A,HangzhouImọ-ẹrọ Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1.
Fi 180μL ti lysozyme buffer si precipitate (dilute the lysozyme to 20mg/mL with lysozyme diluent), pipette lati dapọ daradara, ati ilana ni 37 ° C fun diẹ ẹ sii ju awọn iṣẹju 30. Mu 1.5mL ti RNase / DNase-free tube centrifuge, ati afikun180μL ti iṣakoso rere ati iṣakoso odi ni ọkọọkan.Fi kun10μL ti iṣakoso inu si ayẹwo lati ṣe idanwo, iṣakoso rere, ati iṣakoso odi ni ọkọọkan, ati lo Iyọkuro Acid Nucleic Acid tabi Reagent Purification (YDP302) nipasẹ Tiangen Biotech (Beijing) Co., Ltd. fun isediwon DNA ti o tẹle, ati jọwọ muna tẹle awọn ilana fun lilo fun pato awọn igbesẹ.Lo DNase/RNase H2O fun elution, ati iwọn didun elution ti a ṣe iṣeduro jẹ 100μL.
Aṣayan 2.
Mu 1.5mL ti tube centrifuge ti ko ni RNase/DNase, ki o ṣafikun 200μL ti iṣakoso rere ati iṣakoso odi ni ọkọọkan.Fi kun10μL ti iṣakoso inu si apẹẹrẹ lati ṣe idanwo, iṣakoso rere, ati iṣakoso odi ni ọkọọkan, ati lo Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) ati Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006).Iyọkuro yẹ ki o ṣe ni ibamu pẹlu itọnisọna fun lilo, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
