Dide ti diflicia tanskinni toxin A / B Genefe (C.diff)
Orukọ ọja
HWTS-OOT031A Acid Iwari Iwari ti acidation fun didectidisium withricile Toxin A / B Geneff) (C.diff) (C.diff) (C.diff) (C.diff) (PRRORCEC)
Iwe-ẹri
CE
Onimọraciology
Dide ti difritisinium pipin (CD), Anaerobic ti o ni idaniloju Ainerobic Sporogenic Dide, jẹ ọkan ninu awọn paṣan akọkọ ti o fa awọn akoran ti nsocominal. Ile-iwosan, nipa 15% ~ 25% ti imọran ajẹsara ti o ni ibatan, 50% ~ 75% ti ara conitis ti o ni ibatan ati 9% ti pseudomeranuus ti o fa arun arun (CDI). Dide paplicile jẹ ibajẹ pathogen, pẹlu awọn igara majele ati awọn igara ti ko ni majele.
Ikanni
| Fa | TCDAoriṣi |
| Roox | tcdboriṣi |
| Vic / Hex | Iṣakoso inu |
Awọn ohun elo imọ-ẹrọ
| Ibi ipamọ | ≤-18 ℃ |
| Oju aye | Oṣu mejila 12 |
| Iru apẹrẹ | aṣọ otilu |
| Tt | ≤3 |
| CV | ≤5.0% |
| Lto | 200cfu / ml |
| Alaye pataki | Lo ohun elo yi lati wa awọn parpones iṣan miiran gẹgẹbi Escherisia Coli, Sthovirucoltis, Coravirus Bendicile Dictive ati Ẹlẹ Eniyan DNA, awọn abajade jẹ gbogbo odi. |
| Awọn ohun elo ti o wulo | Lo awọn biosystems 7500 awọn eto PC-akoko akoko-akoko Loo biositystems 7500 iyara awọn eto PC-akoko Iṣiro®5 Awọn ọna PCRE akoko gidi Awọn eto PCR-96P gidi-akoko Ipo ina®480 Eto PRC Linegene 9600 Plut eto iṣawari akoko-akoko PCR (FQD-96a,HanlzhouImọ-ẹrọ Bioer) Ma-6000 Giga iwọn-akoko giga-akoko giga-akoko (Suzhou Molarray Co., Ltd.) Eto PFX96 Akoko PRC Biokad CFX Opus 96 Eto PRC |
Iṣẹ sisan
Aṣayan 1.
Ṣafikun 180μl ti lysozyme Buffer si iṣaju (ti dilute Lysozyme si 20MG / O dara pẹlu diẹ ẹ sii ju iṣẹju 30 ki o si ṣafikun180μL ti iṣakoso rere ati iṣakoso odi ni ọkọọkan. Fikun10μL ti iṣakoso inu si awọn ayẹwo, ati iṣakoso odi, ati lo ifasẹhin odi, ati lo iṣiṣẹ odi Jọwọ tẹle awọn itọnisọna fun lilo fun awọn igbesẹ kan pato. Lo DNNASE / RNASS HE2Ẹyin fun etika, ati iwọn didun igba ti iṣeduro ti wa ni 100μL.
Aṣayan 2.
Mu 1.5ML ti RNAse / DNASE-Free Centrifuced tube, ki o ṣafikun 200μL ti iṣakoso rere ati iṣakoso odi ni ọkọọkan. Fikun10μL ti iṣakoso inu si awọn apẹẹrẹ lati ṣe idanwo, iṣakoso rere, ati lo awọn Macro & bulọọgi-3004-32, HWTS-3004- 96) Ati Makiro & Micro-bulọọgi Lat unclenic acid aifọwọyi (hwts-3006). Ise iseleji yẹ ki o gbe ni ibasọrọ pẹlu itọnisọna fun lilo, ati iwọn didun igba ti o ṣe iṣeduro jẹ 80μL.
