Orientia tsutsugamushi
Orukọ ọja
HWTS-OT002B Orientia tsutsugamushiOhun elo Iwari Acid Nucleic (Pluorescence PCR)
Arun-arun
Scrub typhus jẹ arun febrile nla ti o ṣẹlẹ nipasẹ ikolu Orientia tsutsugamushi (Ot). Orientia scrub typhus jẹ giramu-odi ọranyan microorganism parasitic intracellular. Orientia scrub typhus jẹ ti iwin Orientia ni aṣẹ Rickettsiales, idile Rickettsiaceae, ati iwin Orientia. Scrub typhus wa ni o kun tan nipasẹ awọn geje ti chigger idin ti o nru pathogens. O jẹ ijuwe ti ile-iwosan nipasẹ iba giga lojiji, eschar, lymphadenopathy, hepatosplenomegaly, ati leukopenia ẹjẹ agbeegbe, bbl Ni awọn ọran ti o lewu, o le fa meningitis, ẹdọ ati ikuna kidinrin, ikuna eto-ara pupọ, ati paapaa iku.
ikanni
| FAM | Orientia tsutsugamushi |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | omi ara tuntun |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 idaako / μL |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR gidi-akoko (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Awọn ọna Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Eto PCR-gidi-gidi, BioRad CFX Opus 96 Eto PCR-gidi-gidi |
Sisan iṣẹ
Niyanju isediwon reagent: Makiro & Micro-IdanwoGbogboogboApo DNA/RNA (HWTS-3019) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-EQ011)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Awọn isediwon yẹ ki o wa ni o waiye ni ibamu si awọn ilana fun lilo ti yi isediwon reagent. Iwọn ayẹwo ti o jade jẹ 200µL, ati iwọn didun elution ti a ṣeduro jẹ100µL.
