Apapọ Awọn ọlọjẹ atẹgun
Orukọ ọja
HWTS-RT183-Ẹni-ẹmi-ẹjẹ Pathogens Apapọ Apo-iwari (Fluorescence PCR)
Arun-arun
Arun Iwoye Corona 2019, tọka si bi 'COVID-19', tọka si ẹdọforo ti o fa nipasẹ ikolu SARS-CoV-2. SARS-CoV-2 jẹ coronavirus ti o jẹ ti iwin β. COVID-19 jẹ arun ajakalẹ-arun ti atẹgun nla, ati pe olugbe ni ifaragba gbogbogbo. Ni lọwọlọwọ, orisun ti akoran jẹ pataki awọn alaisan ti o ni akoran nipasẹ 2019-nCoV, ati pe awọn eniyan ti o ni akoran asymptomatic le tun di orisun ti akoran. Da lori iwadii ajakale-arun lọwọlọwọ, akoko isubu jẹ awọn ọjọ 1-14, pupọ julọ awọn ọjọ 3-7. Iba, Ikọaláìdúró gbigbẹ ati rirẹ jẹ awọn ifarahan akọkọ. Awọn alaisan diẹ ni awọn aami aiṣan bii isunmi imu, imu imu, ọfun ọfun, myalgia ati igbuuru, ati bẹbẹ lọ. O ti wa ni gíga àkóràn. O ti wa ni o kun tan nipasẹ iwúkọẹjẹ ati sneezing. O maa n jade ni orisun omi ati igba otutu. Awọn ọlọjẹ aarun ayọkẹlẹ ti pin si aarun ayọkẹlẹ A (IFV A), aarun ayọkẹlẹ B (IFV B), ati aarun ayọkẹlẹ C (IFV C) awọn oriṣi mẹta, gbogbo wọn jẹ ti ọlọjẹ alalepo, ti o fa arun eniyan ni pataki fun awọn ọlọjẹ A ati B, o jẹ ọlọjẹ RNA kan ti o ni okun, ti o pin si. Kokoro aarun ayọkẹlẹ jẹ akoran ti atẹgun nla, pẹlu H1N1, H3N2 ati awọn ẹya-ara miiran, eyiti o ni itara si iyipada ati ibesile agbaye. 'Shift' n tọka si iyipada ti ọlọjẹ aarun ayọkẹlẹ A, ti o yorisi ifarahan ti ọlọjẹ tuntun 'subtype'. Awọn ọlọjẹ aarun ayọkẹlẹ B ti pin si awọn idile meji, Yamagata ati Victoria Influenza virus nikan ni o ni fifo antigenic, ati pe o yago fun eto iwo-kakiri ati imukuro eto ajẹsara eniyan nipasẹ iyipada rẹ. Sibẹsibẹ, iyara itankalẹ ti ọlọjẹ aarun ayọkẹlẹ B jẹ o lọra ju ti ọlọjẹ aarun ayọkẹlẹ eniyan A. Kokoro aarun ayọkẹlẹ B tun le fa awọn akoran atẹgun eniyan ati ja si awọn ajakale-arun.
Kokoro syncytial ti atẹgun (RSV) jẹ ọlọjẹ RNA kan, ti o jẹ ti idile paramyxoviridae. O ti gbejade nipasẹ awọn isunmi afẹfẹ ati isunmọ isunmọ ati pe o jẹ pathogen akọkọ ti ikolu ti atẹgun atẹgun ninu awọn ọmọde. Awọn ọmọde ti o ni RSV le ni idagbasoke bronchiolitis ti o lagbara ati pneumonia, eyiti o ni ibatan si ikọ-fèé ninu awọn ọmọde. Awọn ọmọ ikoko ni awọn aami aiṣan ti o lagbara, pẹlu iba giga, rhinitis, pharyngitis ati laryngitis, ati lẹhinna bronchiolitis ati pneumonia. Awọn ọmọde ti o ni aisan diẹ le ni idiju pẹlu otitis media, pleurisy ati myocarditis, bbl
Imọ paramita
| Ibi ipamọ | -18 ℃ Ninu okunkun |
| Selifu-aye | osu 9 |
| Apeere Iru | Oropharyngeal swab; Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| LoD | 200 idaako / μL |
| Ni pato | Awọn abajade ifasilẹ-agbelebu fihan pe ko si ifaseyin agbelebu laarin ohun elo ati cytomegalovirus, ọlọjẹ herpes simplex iru 1, ọlọjẹ varicella zoster, ọlọjẹ Epstein-Barr, adenovirus, metapneumovirus eniyan, rhinovirus, ọlọjẹ parainfluenza iru I/II/III/IV, bocavirus, enterovirus, coronavirus, Chhhemiaudiamonia p. Bordetella pertussis, Corynebacterium spp., Escherichia coli, Haemophilus influenzae, Lactobacillus spp., Legionella pneumophila, Moraxella catarrhalis, attenuated igara ti Mycobacterium iko, Neisseria meningitidis, Neisseria spp. Streptococcus pneumoniae, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardia, Serratia marcespercoccus, Cryptoccus, fumigatus, Aspergillus flavus, Pneumocystis carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q iba ati eda eniyan genomic nucleic acid. |
| Awọn ohun elo ti o wulo | Ohun elo Biosystems 7500 Real-akoko PCR Systems, Applied Biosystems 7500 Yara gidi-akoko PCR Systems, QuantStudio®5 Awọn ọna PCR-gidi-gidi, SLAN-96P Awọn ọna PCR-akoko gidi (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX96 Real-Time PCR System, O PCR-Time Real Time System-CFX96 |
Sisan iṣẹ
Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (eyiti o le ṣee lo pẹlu Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), ati Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8 le ṣee lo)TM AIO800 (HWTS-EQ007)) nipasẹ Jiangsu Makiro & Micro-Test Med-Tech Co., Ltd.
Iwọn ayẹwo ti o jade jẹ 200μL ati iwọn didun elution ti a ṣe iṣeduro jẹ 150μL.
