SARS-CoV-2/aarun ayọkẹlẹ A / aarun ayọkẹlẹ B
Orukọ ọja
HWTS-RT148-SARS-CoV-2/aarun ayọkẹlẹ A / aarun ayọkẹlẹ B Nucleic Acid Apo Iwari (Pluorescence PCR)
ikanni
| Orukọ ikanni | PCR-Idapọ 1 | PCR-Idapọ 2 |
| FAM ikanni | ORF1ab pupọ | IVA |
| VIC / HEX ikanni | Iṣakoso inu | Iṣakoso inu |
| CY5 ikanni | N jiini | / |
| ROX ikanni | E jiini | IVB |
Imọ paramita
| Ibi ipamọ | -18 ℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | nasopharyngeal swabs ati oropharyngeal swabs |
| Àfojúsùn | SARS-CoV-2 awọn ibi-afẹde mẹta (Orf1ab, N ati awọn Jiini E) / aarun ayọkẹlẹ A / aarun ayọkẹlẹ B |
| Ct | ≤38 |
| CV | ≤10.0% |
| LoD | SARS-CoV-2: 300 idaako/ml aarun ayọkẹlẹ A: 500 idaako/ml aarun ayọkẹlẹ B kokoro: 500 idaako/ml |
| Ni pato | a) Awọn abajade idanwo agbelebu fihan pe ohun elo naa ni ibamu pẹlu coronavirus eniyan SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, ọlọjẹ syncytial atẹgun A ati B, ọlọjẹ parainfluenza 1, 2 ati 3, rhinovirusA, B ati C, adenovirus 1, 2, 3, 4, 5, 7 and 55, human metapneumovirus, enterovirus A, B, C and D, virus cytoplasmic pulmonary virus, EB virus, measles virus Human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pneumoniae, Streptococcus pneumoniae, mygacoccus, mygacoccus pneumonia, mygacoccus, mygacoccus. Candida albicans, Candida glabrata Ko si irekọja laarin Pneumocystis yersini ati Cryptococcus neoformans. b) Agbara kikọlu alatako: yan mucin (60mg/mL), 10% (V/V) ẹjẹ eniyan, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), iṣuu soda kiloraidi (ti o ni itọju) (20mg/mL), beclomethasone (20mg/ml), dexamethasone (20mg/mL), flunisone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/ml), α-Interferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), pramivir (1mg/mL), lopinavir (500mg/mL). ritonavir (60mg/ml), mupirocin (20mg/mL), azithromycin (1mg/mL), ceprotene (40μg/mL) Meropenem (200mg/mL), levofloxacin (10μg/ml) ati tobramycin (0.6mg/mL) .Awọn abajade fihan pe awọn nkan ti o ni idiwọ ni awọn ifọkansi ti o wa loke ko ni idahun kikọlu si awọn abajade wiwa ti awọn ọlọjẹ. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems SLAN ®-96P Real-Time PCR Systems QuantStudio™ 5 Awọn ọna PCR-gidi-gidi LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Lapapọ PCR Solusan
