SARS-CoV-2/aarun ayọkẹlẹ A / aarun ayọkẹlẹ B
Orukọ ọja
HWTS-RT148-SARS-CoV-2/aarun ayọkẹlẹ A / aarun ayọkẹlẹ B Nucleic Acid Apo Iwari Ohun elo (Fluorescence PCR)
ikanni
| Orukọ ikanni | PCR-Idapọ 1 | PCR-Idapọ 2 |
| FAM ikanni | ORF1ab pupọ | IVA |
| VIC / HEX ikanni | Iṣakoso inu | Iṣakoso inu |
| CY5 ikanni | N jiini | / |
| ROX ikanni | E jiini | IVB |
Imọ paramita
| Ibi ipamọ | -18 ℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | nasopharyngeal swabs ati oropharyngeal swabs |
| Àfojúsùn | SARS-CoV-2 awọn ibi-afẹde mẹta (Orf1ab, N ati awọn Jiini E) / aarun ayọkẹlẹ A / aarun ayọkẹlẹ B |
| Ct | ≤38 |
| CV | ≤10.0% |
| LoD | SARS-CoV-2: 300 idaako/ml aarun ayọkẹlẹ A: 500 idaako/ml aarun ayọkẹlẹ B kokoro: 500 idaako/ml |
| Ni pato | a) Awọn abajade idanwo agbelebu fihan pe ohun elo naa ni ibamu pẹlu coronavirus eniyan SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, ọlọjẹ syncytial ti atẹgun A ati B, ọlọjẹ parainfluenza 1, 2 ati 3, rhinovirus, 3 virus, ade2virus, rhinovirus, ade2virus. 5, 7 ati 55, eniyan metapneumovirus, enterovirus A, B, C ati D, eniyan cytoplasmic ẹdọforo kokoro, EB virus, measles virus Human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia, Pneumoniae, Lemunofila. aarun ayọkẹlẹ, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium iko, Aspergillus fumigatus, Candida albicans, Candida glabrata Ko si ifaseyin agbelebu laarin Pneumocystis yersinioform ati Cryptoans. b) Agbara kikọlu alatako: yan mucin (60mg/mL), 10% (V/V) ẹjẹ eniyan, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), iṣuu soda kiloraidi (ti o ni itọju) (20mg/ml), beclomethasone (20mg/mL), dexamethasm2 (20mg/mL0), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/mL), ribavirin (10mg/mL), (1mg / mL), lopinavir (500mg / mL), ritonavir (60mg / mL), mupirocin (20mg / mL), azithromycin (1mg / ml), ceprotene (40μg / ml) Meropenem (200mg / ml), levofloxacin (1.6 tom / Lmcin) ati 0. Awọn abajade fihan pe awọn nkan ti o ni idiwọ ni awọn ifọkansi ti o wa loke ko ni idahun kikọlu si awọn abajade wiwa ti awọn ọlọjẹ. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems SLAN ®-96P Real-Time PCR Systems QuantStudio™ 5 Awọn ọna PCR-gidi-gidi LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Lapapọ PCR Solusan
