Yersinia Pestis Nucleic Acid
Orukọ ọja
HWTS-OT014-Yersinia Pestis Ohun elo Iwari Acid Nucleic Acid (Fluorescence PCR)
Arun-arun
Yersinia pestis, ti a mọ ni Yersinia pestis, ṣe atunṣe ni kiakia ati pe o ni ipalara ti o ga, eyiti o jẹ kokoro arun ti o wọpọ ti ajakale-arun laarin awọn eku ati ajakalẹ-arun laarin awọn eniyan. Awọn ipa ọna akọkọ mẹta wa ti gbigbe: ① gbigbe nipasẹ awọ ara: ikolu nipasẹ awọ ti o bajẹ ati awọn membran mucous nitori olubasọrọ pẹlu sputum alaisan ati pus ti o ni kokoro arun, tabi pẹlu awọ ẹranko, ẹjẹ, ẹran, ati pẹlu awọn igbe ti awọn fleas ajakale-arun; ② gbigbe nipasẹ ọna ti ngbe ounjẹ: ikolu nipasẹ ọna ti ngbe ounjẹ nitori jijẹ awọn ẹranko ti a ti doti; ③ gbigbe nipasẹ ọna atẹgun: sputum ti o ni kokoro arun, droplets tabi eruku ti o tan nipasẹ awọn isunmi atẹgun, fa ajakaye-arun laarin eniyan. Awọn ajakalẹ-arun pataki mẹta ti ajakalẹ-arun ti wa ninu itan-akọọlẹ eniyan, akọkọ jẹ “Arun ti Justinian” ni ọrundun 6th; atẹle nipa "Iku Dudu" ti o pa fere 1/3 ti awọn olugbe Europe ni ọdun 14th; Ajakaye-arun kẹta bẹrẹ ni agbegbe Yunnan, China ni ọrundun 19th, lẹhinna gba kọja guusu China o tan kaakiri si Ilu Họngi Kọngi ati paapaa agbaye. Lakoko awọn ajakalẹ-arun mẹta wọnyi, diẹ sii ju 100 milionu eniyan padanu ẹmi wọn.
Imọ paramita
| Ibi ipamọ | -18 ℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Ọfun swab |
| CV | ≤5.0% |
| LoD | 500 idaako / μL |
| Awọn ohun elo ti o wulo | O wulo lati tẹ reagent iwari I: Ohun elo Biosystems 7500 Real-akoko PCR Systems, QuantStudio®5 Awọn ọna PCR akoko gidi, SLAN-96P Awọn ọna PCR-gidi-gidi (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Awọn ọna Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer), MA-6000 Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Eto PCR akoko-gidi, BioRad CFX Opus 96 Real-Time PCR System. Wulo fun iru II reagent iwari: EudemonTMAIO800 (HWTS-EQ007) nipasẹ Jiangsu Makiro & Micro-Test Med-Tech Co., Ltd. |
Sisan iṣẹ
Awọn Makiro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. le ṣee lo pẹlu Makiro & Micro-Cestcid Automatic NucleicC3 HWTS-3006B). Awọn isediwon yẹ ki o wa ni o waiye ni ibamu si awọn ilana fun lilo. Iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
