Zaire Ebola Iwoye
Orukọ ọja
HWTS-FE008 Zaire Ebola Iwoye Ohun elo Iwari Acid Acid (Fluorescence PCR)
Arun-arun
Kokoro Ebola jẹ ti Filoviridae, eyiti o jẹ ọlọjẹ RNA odi-okun odi-okun ti ko ni ipin.Awọn ọlọjẹ jẹ awọn filaments gigun pẹlu aropin virion ipari ti 1000nm ati iwọn ila opin kan ti o to 100nm.Jiini ọlọjẹ Ebola jẹ RNA odi-okun ti ko ni ipin pẹlu iwọn 18.9kb, fifi koodu pa awọn ọlọjẹ igbekalẹ 7 ati amuaradagba ti kii ṣe igbekalẹ 1.Kokoro Ebola le pin si awọn oriṣi bii Zaire, Sudan, Bundibugyo, Tai Forest ati Reston.Lara wọn, iru Zaire ati iru Sudan ni a ti royin lati fa iku ọpọlọpọ eniyan lati ikolu.EHF (Iba Ẹjẹ Ẹjẹ Ẹjẹ Ebola) jẹ arun ajakalẹ-ẹjẹ ẹjẹ ti o ga ti o fa nipasẹ ọlọjẹ Ebola.Awọn eniyan ni o ni akoran nipataki nipasẹ olubasọrọ pẹlu awọn omi ara, awọn aṣiri ati excreta ti awọn alaisan tabi awọn ẹranko ti o ni akoran, ati awọn ifihan ile-iwosan jẹ iba ti o jade ni pataki, ẹjẹ ati ibajẹ ara eniyan pupọ.EHF ni oṣuwọn iku giga ti 50% -90%.
ikanni
| FAM | MP nucleic acid |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | Omi ara tuntun, pilasima |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 idaako / μL |
| Ni pato | Lo awọn ohun elo lati ṣe idanwo awọn itọkasi odi ile-iṣẹ, awọn abajade pade awọn ibeere. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR gidi-akoko (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Eto Wiwa PCR Akoko-gidi(FQD-96A, imọ-ẹrọ Hangzhou Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Eto PCR-gidi-gidi, ati BioRad CFX Opus 96 Eto PCR-gidi-gidi |
Sisan iṣẹ
Aṣayan 1.
Niyanju isediwon reagent: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid isediwon tabi ìwẹnumọ Reagent (YDP315-R) nipa Tiangen Biotech (Beijing) Co., Ltd.O yẹ ki o fa jade ni ibamu si awọn itọnisọna, ati iwọn didun isediwon ti a ṣe iṣeduro jẹ 140μL ati iwọn didun elution ti a ṣe iṣeduro jẹ 60μL.
Aṣayan 2.
Niyanju isediwon reagent: Makiro & Micro-Igbeyewo Gbogun ti DNA/RNA Apo (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor (HWTS-3006) .It. yẹ ki o fa jade ni ibamu si awọn ilana.Iwọn ayẹwo isediwon jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
Aṣayan 3.
Niyanju isediwon reagents: Nucleic Acid Reagent isediwon (1000020261) ati High Nipasẹput aládàáṣiṣẹ Ayẹwo igbaradi System (MGISP-960) nipa BGI yẹ ki o wa jade ni ibamu si awọn ilana.Iwọn isediwon jẹ 160μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 60μL.
