Awọn oriṣi 14 ti Papillomavirus eniyan ti o ni eewu giga (16/18/52 Titẹ)
Orukọ ọja
HWTS-CC019A-Awọn oriṣi 14 ti Papillomavirus Eniyan ti o ni eewu giga (Titẹ 16/18/52) Apo Iwari Acid Nucleic (Pluorescence PCR)
Arun-arun
Awọn ẹkọ-ẹkọ ti fihan pe awọn akoran ti o tẹsiwaju HPV ati ọpọlọpọ awọn akoran jẹ ọkan ninu awọn okunfa akọkọ ti akàn ti ara.Lọwọlọwọ, awọn itọju ti o munadoko ti a mọ si tun ko ni fun akàn cervical ti o fa nipasẹ HPV, nitorinaa wiwa ni kutukutu ati idena ti akoran oyun ti o fa nipasẹ HPV jẹ bọtini lati ṣe idiwọ akàn cervical.O ṣe pataki pupọ lati fi idi kan ti o rọrun, kan pato ati idanwo idanimọ etiology iyara fun iwadii ile-iwosan ati itọju ti akàn cervical.
ikanni
| ikanni | Iru |
| FAM | HPV 18 |
| VIC/HEX | HPV 16 |
| ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY5 | HPV 52 |
| Kúsari 705/CY5.5 | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Ito, Swab cervical, Obo swab |
| Ct | ≤28 |
| LoD | 300 idaako/ml |
| Ni pato | Ko si ifaseyin agbelebu pẹlu awọn ayẹwo atẹgun miiran gẹgẹbi Aarun ayọkẹlẹ A, Aarun ayọkẹlẹ B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Virus Syncytial Respiratory, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneu2 virus B1/B2, Kokoro syncytial ti atẹgun A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, bbl ati DNA genomic eniyan. |
| Awọn ohun elo ti o wulo | MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Eto PCR akoko-gidi ati BioRad CFX Opus 96 Eto PCR akoko-gidi |
Sisan iṣẹ
1.Apeere ito
A: Gba1.4milimita ti ito ayẹwo lati ṣe idanwo ati centrifuge ni 12000rpm fun awọn iṣẹju 5;Jabọ supernatant (o gba ọ niyanju lati tọju 10-20μL supernatant lati isalẹ ti tube centrifuge), ṣafikun 200μL ti reagent itusilẹ ayẹwo, ati isediwon atẹle yẹ ki o ṣe ni ibamu si awọn ilana fun lilo Macro & Micro-Test Ayẹwo Tu silẹ Reagent (HWTS-3005-8).
B: gba1.4milimita ti ito ayẹwo lati ṣe idanwo ati centrifuge ni 12,000rpm fun awọn iṣẹju 5;yọọ kuro (a ṣe iṣeduro lati tọju 10-20μL ti supernatant lati isalẹ ti tube centrifuge), ki o si fi 200μL ti saline deede lati tun pada, bi apẹẹrẹ lati ṣe idanwo.Iyọkuro ti o tẹle le ṣee ṣe pẹlu Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & amupu; Idanwo Micro-Aifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ni ibamu pẹlu itọnisọna naa.s fun lilo.Iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
C: Gba1.4milimita ti ito ayẹwo lati ṣe idanwo ati centrifuge ni 12,000rpm fun awọn iṣẹju 5;yọọ kuro (a ṣe iṣeduro lati tọju 10-20μL ti supernatant lati isalẹ ti tube centrifuge), ki o si fi 200μL ti saline deede lati tun pada, bi apẹẹrẹ lati ṣe idanwo.Iyọkuro ti o tẹle le ṣee ṣe pẹluQIAamp DNA Mini Kit(51304) nipasẹ QIAGEN tabi Makiro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Awọn isediwon yẹ ki o wa ni ilọsiwaju ni ibamu si awọn ilana fun lilo.Iwọn ayẹwo isediwon jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
2. Ayẹwo cervical swab / obo swab
A: Mu 1mL ti ayẹwo lati ṣe idanwo ni 1.5mL kanof tube centrifuge,aticentrifuge ni 12000rpm fun iṣẹju 5. Discard supernatant (o gba ọ niyanju lati tọju 10-20μL ti supernatant lati isalẹ ti tube centrifuge), ṣafikun 100μL ti reagent itusilẹ ayẹwo, lẹhinna jade ni ibamu si awọn ilana fun lilo Makiro & Micro-Test Ayẹwo Tu Reagent ( HWTS-3005-8).
B: Awọn isediwon le ṣee waiye pẹlu Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro. & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ni ibamu pẹlu awọn ilana fun lilo.Iwọn ayẹwo ti o jade jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
C: Iyọkuro le ṣee ṣe pẹlu QIAamp DNA Mini Kit (51304) nipasẹ QIAGEN tabi Makiro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Awọn isediwon yẹ ki o wa ni ilọsiwaju ni ibamu si awọn ilana fun lilo.Iwọn ayẹwo isediwon jẹ 200 μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ80 μL.
3, Swab cervical/ Obo Swab
Ṣaaju ki o to ṣe ayẹwo, lo swab owu lati rọra nu awọn aṣiri ti o pọ julọ lati cervix, ki o si lo swab owu miiran ti a fi sinu pẹlu ojutu itọju sẹẹli tabi fẹlẹ iṣapẹẹrẹ sẹẹli exfoliated cervical lati faramọ mucosa cervical ati ki o yipada ni aago 3-5 yika lati gba. awọn sẹẹli exfoliated cervical.Mu rọra yọ swab owu tabi fẹlẹ,atifi sinu tube ayẹwo pẹlu 1mL ti iyọ deede ti o ni ifo ilera. ALẹhin ti o ba fi omi ṣan ni kikun, fun pọ gbẹ swab owu tabi fẹlẹ si ogiri tube ki o sọ ọ silẹ, mu fila tube naa pọ, ki o samisi orukọ apẹẹrẹ (tabi nọmba) ki o tẹ sori tube ayẹwo.
