Awọn oriṣi 28 ti Iwoye Papilloma Eniyan ti o ni eewu giga (Titẹ 16/18) Acid Nucleic

Awọn oriṣi 28 ti Iwoye Papilloma Eniyan ti o ni eewu giga (Titẹ 16/18) Aworan Afihan Acid Nucleic

Apejuwe kukuru:

Ohun elo yii dara fun wiwa agbara in vitro ti awọn oriṣi 28 ti awọn ọlọjẹ papilloma eniyan (HPV) (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 5,5,5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 45, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 45, 45, 5, 5, 5, 5, 45). 61, 66, 68, 73, 81, 82, 83) acid nucleic ninu ito ọkunrin / obinrin ati awọn sẹẹli exfoliated cervical abo. HPV 16/18 le ti wa ni titẹ, awọn iru ti o ku ko le ṣe titẹ patapata, pese awọn ọna iranlọwọ fun ayẹwo ati itọju ti ikolu HPV.

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Orukọ ọja

HWTS-CC006A-28 Awọn oriṣi ti Iwoye Papilloma Eniyan ti o ni eewu giga (Titẹ 16/18) Ohun elo Iwari Acid Nucleic (Fluorescence PCR)

Arun-arun

Akàn jẹ ọkan ninu awọn èèmọ buburu ti o wọpọ julọ ti apa ibisi obinrin. Awọn ijinlẹ ti fihan pe awọn akoran itẹramọṣẹ HPV ati ọpọlọpọ awọn akoran jẹ ọkan ninu awọn okunfa akọkọ ti akàn ti ara. Lọwọlọwọ, awọn itọju ti o munadoko ti a mọ si tun ko ni fun akàn cervical ti o fa nipasẹ HPV, nitorinaa wiwa ni kutukutu ati idena ti akoran oyun ti o fa nipasẹ HPV jẹ bọtini lati ṣe idiwọ akàn cervical. O jẹ iwulo nla lati fi idi kan ti o rọrun, pato ati idanwo idanimọ etiology iyara fun ayẹwo ile-iwosan ati itọju ti akàn cervical.

ikanni

Apapo lenu	ikanni	Iru
PCR-Mix1	FAM	18
	VIC(HEX)	16
	ROX	31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68
	CY5	Iṣakoso ti abẹnu
PCR-Mix2	FAM	6, 11, 54, 83
	VIC(HEX)	26, 44, 61, 81
	ROX	40, 42, 43, 53, 73, 82
	CY5	Iṣakoso ti abẹnu

Imọ paramita

Ibi ipamọ	Omi: ≤-18℃
Selifu-aye	12 osu
Apeere Iru	Swab cervical, Swab abẹ, ito
Ct	≤28
CV	<5.0%
LoD	300 idaako/ml
Awọn ohun elo ti o wulo	Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio^®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler^®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System

Sisan iṣẹ

Niyanju isediwon reagent: Makiro & Micro-igbeyewo Gbogun ti DNA/RNA Apo (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-West laifọwọyi Nucleic Acid Extractor (30H6) HWTS-3006B))) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ṣafikun 200μL ti saline deede lati tun da pellet pada ni igbesẹ 2.1, ati lẹhinna isediwon yẹ ki o ṣe ni ibamu si awọn ilana fun lilo reagent isediwon yii. Iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
Niyanju isediwon reagent: QIAamp DNA Kit Mini Kit (51304) tabi Makiro & Micro-Test Viral DNA/RNA Ọwọn (HWTS-3020-50). Ṣafikun 200μL ti iyọ deede lati tun pada pellet ni igbese 2.1, ati lẹhinna isediwon yẹ ki o waiye ni ibamu si awọn ilana fun lilo reagent isediwon yii. Iwọn ayẹwo ti o jade ti awọn ayẹwo jẹ gbogbo 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 100μL.

Ti tẹlẹ: Enterococcus-sooro Vancomycin ati Gene-sooro Oògùn

Itele: Eda eniyan Papillomavirus (28 Orisi) Genotyping

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