Awọn oriṣi 28 ti Iwoye Papilloma Eniyan ti o ni eewu giga (Titẹ 16/18) Acid Nucleic
Orukọ ọja
HWTS-CC006A-28 Awọn oriṣi ti Iwoye Papilloma Eniyan ti o ni eewu giga (Titẹ 16/18) Ohun elo Iwari Acid Nucleic (Fluorescence PCR)
Arun-arun
Akàn jẹjẹ ọkan ninu awọn èèmọ buburu ti o wọpọ julọ ti apa ibisi obinrin.Awọn ẹkọ-ẹkọ ti fihan pe awọn akoran ti o tẹsiwaju HPV ati ọpọlọpọ awọn akoran jẹ ọkan ninu awọn okunfa akọkọ ti akàn ti ara.Lọwọlọwọ, awọn itọju ti o munadoko ti a mọ si tun ko ni fun akàn cervical ti o fa nipasẹ HPV, nitorinaa wiwa ni kutukutu ati idena ti akoran oyun ti o fa nipasẹ HPV jẹ bọtini lati ṣe idiwọ akàn cervical.O ṣe pataki pupọ lati fi idi kan ti o rọrun, kan pato ati idanwo idanimọ etiology iyara fun iwadii ile-iwosan ati itọju ti akàn cervical.
ikanni
| Apapo lenu | ikanni | Iru |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Iṣakoso ti abẹnu | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | Omi: ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Swab cervical, Swab abẹ, ito |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300 idaako/ml |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Niyanju isediwon reagent: Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Igbeyewo. Laifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Fi 200μL ti saline deede lati tun pada pellet ni igbese 2.1, ati lẹhinna isediwon yẹ ki o wa ni ibamu pẹlu si awọn ilana fun lilo ti yi reagent isediwon.Iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
Niyanju isediwon reagent: QIAamp DNA Kit Mini Kit (51304) tabi Makiro & Micro-Test Viral DNA/RNA Ọwọn (HWTS-3020-50).Ṣafikun 200μL ti iyọ deede lati tun pada pellet ni igbese 2.1, ati lẹhinna isediwon yẹ ki o waiye ni ibamu si awọn ilana fun lilo reagent isediwon yii.Iwọn ayẹwo ti awọn ayẹwo jẹ gbogbo 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 100μL.
