Iwoye Dengue, Kokoro Zika ati Chikungunya Iwoye Multiplex
Orukọ ọja
HWTS-FE040 Iwoye Dengue, Kokoro Zika ati Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Pluorescence PCR)
Arun-arun
Ibà dengue (DF), eyiti o fa nipasẹ kokoro arun dengue (DENV), jẹ ọkan ninu awọn arun ajakale-arun arbovirus julọ. Alabọde gbigbe rẹ pẹlu Aedes aegypti ati Aedes albopictus. DF jẹ eyiti o wọpọ julọ ni awọn agbegbe otutu ati awọn agbegbe iha ilẹ. DENV jẹ ti flavivirus labẹ flaviviridae, ati pe o le pin si awọn serotypes 4 ni ibamu si antijeni oju. Awọn ifarahan ile-iwosan ti DENV ni pataki pẹlu orififo, iba, ailera, imugboroja ti apo-ara-ara-ara, leukopenia ati bẹbẹ lọ, ati ẹjẹ, ipaya, ipalara ẹdọ tabi paapaa iku ni awọn iṣẹlẹ ti o lagbara. Ni awọn ọdun aipẹ, iyipada oju-ọjọ, ilu ilu, idagbasoke iyara ti irin-ajo ati awọn ifosiwewe miiran ti pese iyara diẹ sii ati irọrun fun gbigbe ati itankale DF, ti o yori si imugboroja igbagbogbo ti agbegbe ajakale-arun ti DF.
ikanni
| FAM | DENV acid nucleic |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | -18 ℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | Omi ara tuntun |
| Ct | ≤38 |
| CV | .5% |
| LoD | 500 idaako/ml |
| Ni pato | Awọn abajade idanwo kikọlu fihan pe nigbati ifọkansi ti bilirubin ninu omi ara ko ju 168.2μmol/ml, ifọkansi haemoglobin ti a ṣe nipasẹ hemolysis ko ju 130g/L, ifọkansi ọra ẹjẹ ko ju 65mmol/ml, apapọ ifọkansi IgG ninu omi ara ko ju 5mg/mL lọ, ko si ni ipa lori kokoro arun chikun. Kokoro Hepatitis A, Kokoro Hepatitis B, ọlọjẹ Hepatitis C, ọlọjẹ Herpes, ọlọjẹ equine encephalitis ti oorun, Hantavirus, kokoro Bunya, ọlọjẹ West Nile ati awọn ayẹwo omi ara eniyan ni a yan fun idanwo ifasilẹ-agbelebu, ati awọn abajade fihan pe ko si ifaseyin agbelebu laarin ohun elo yii ati awọn ọlọjẹ ti a mẹnuba loke. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1.
TIANAmp Iwoye DNA/RNA Kit (YDP315-R), ati isediwon yẹ ki o wa ni o waiye ni ti o muna ibamu pẹlu awọn ilana fun lilo. Iwọn ayẹwo ti o jade jẹ 140μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 60μL.
Aṣayan 2.
Makiro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Igbeyewo Aifọwọyi Nucleic Acid Extractor (HWTS-3006C) & TS 030 C. Micro-Test Med-Tech Co., Ltd., ati isediwon yẹ ki o ṣe ni ibamu si itọnisọna fun lilo. Iwọn ayẹwo ti o jade jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
