Iwoye Dengue, Kokoro Zika ati Chikungunya Iwoye Multiplex
Orukọ ọja
HWTS-FE040 Iwoye Dengue, Kokoro Zika ati Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Pluorescence PCR)
Arun-arun
Ibà dengue (DF), eyiti o fa nipasẹ kokoro arun dengue (DENV), jẹ ọkan ninu awọn arun ajakale-arun arbovirus julọ.Alabọde gbigbe rẹ pẹlu Aedes aegypti ati Aedes albopictus.DF jẹ eyiti o wọpọ julọ ni awọn agbegbe otutu ati awọn agbegbe iha ilẹ.DENV jẹ ti flavivirus labẹ flaviviridae, ati pe o le pin si awọn serotypes 4 ni ibamu si antijeni oju.Awọn ifarahan ile-iwosan ti ikolu DENV ni akọkọ pẹlu orififo, iba, ailera, imugboroja ti apo-ara-ara, leukopenia ati bẹbẹ lọ, ati ẹjẹ, ipaya, ipalara ẹdọ tabi paapaa iku ni awọn iṣẹlẹ ti o lagbara.Ni awọn ọdun aipẹ, iyipada oju-ọjọ, ilu ilu, idagbasoke iyara ti irin-ajo ati awọn ifosiwewe miiran ti pese iyara diẹ sii ati awọn ipo irọrun fun gbigbe ati itankale DF, ti o yori si imugboroosi igbagbogbo ti agbegbe ajakale-arun ti DF.
ikanni
| FAM | MP nucleic acid |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | -18 ℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | Omi ara tuntun |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 idaako/ml |
| Ni pato | Awọn abajade idanwo kikọlu fihan pe nigbati ifọkansi ti bilirubin ninu omi ara ko ju 168.2μmol / milimita, ifọkansi haemoglobin ti a ṣe nipasẹ hemolysis ko ju 130g/L, ifọkansi ọra ẹjẹ ko ju 65mmol/ml, lapapọ IgG ifọkansi ninu omi ara ko ju 5mg/mL lọ, ko si ipa lori ọlọjẹ dengue, ọlọjẹ Zika tabi wiwa ọlọjẹ chikungunya.Kokoro Hepatitis A, Kokoro Ẹdọgba B, Kokoro Hepatitis C, Kokoro Herpes, ọlọjẹ Equine encephalitis Ila-oorun, Hantavirus, Kokoro Bunya, Kokoro West Nile ati awọn ayẹwo omi ara eniyan ni a yan fun idanwo ifasilẹ-agbelebu, ati awọn abajade fihan pe ko si. irekọja laarin kit yii ati awọn pathogens ti a mẹnuba loke. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1.
TIANAmp Iwoye DNA/RNA Kit (YDP315-R), ati isediwon yẹ ki o wa ni o waiye ni ti o muna ibamu pẹlu awọn ilana fun lilo.Iwọn ayẹwo ti o jade jẹ 140μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 60μL.
Aṣayan 2.
Makiro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Igbeyewo Aifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., ati isediwon yẹ ki o waiye ni ibamu si awọn ilana fun lilo.Iwọn ayẹwo ti o jade jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
