Didi-si dahùn o Zaire ati Sudan Ebolavirus Nucleic Acid
Orukọ ọja
HWTS-FE035-Didi-si dahùn o Zaire ati Sudan Ebolavirus Nucleic Acid Apo (Fluorescence PCR)
Arun-arun
Ebolavirus jẹ ti Filoviridae, eyiti o jẹ ọlọjẹ RNA odi-okun odi-okun ti ko ni ipin. Awọn ọlọjẹ jẹ awọn filaments gigun pẹlu aropin virion ipari ti 1000nm ati iwọn ila opin kan ti o to 100nm. Jiini Ebolavirus jẹ RNA odi-okun odi ti ko ni ipin pẹlu iwọn 18.9kb, fifi koodu pa awọn ọlọjẹ igbekalẹ 7 ati amuaradagba ti kii ṣe igbekalẹ 1. Ebola le pin si awọn oriṣi bii Zaire, Sudan, Bundibugyo, Tai Forest ati Reston. Lara wọn, iru Zaire ati iru Sudan ni a ti royin lati fa iku ọpọlọpọ eniyan lati ikolu. EHF (Ìbà Ẹjẹ Ẹjẹ ti Ebola) jẹ arun ajakalẹ-ẹjẹ-ẹjẹ nla ti o fa nipasẹ Ebola. Awọn eniyan ni o ni akoran nipataki nipasẹ olubasọrọ pẹlu awọn omi ara, awọn aṣiri ati excreta ti awọn alaisan tabi awọn ẹranko ti o ni akoran, ati awọn ifihan ile-iwosan jẹ iba ti o jade ni pataki, ẹjẹ ati ibajẹ ara eniyan pupọ. EHF ni oṣuwọn iku giga ti 50% -90%. Ni lọwọlọwọ, awọn ọna ti iwadii Ebolavirus jẹ awọn idanwo yàrá ni pataki, pẹlu awọn abala meji: wiwa etiological ati wiwa serological. Wiwa etiological pẹlu wiwa awọn antigens gbogun ninu awọn ayẹwo ẹjẹ nipasẹ ELISA, wiwa awọn acids nucleic nipasẹ awọn ọna imudara bii RT-PCR, ati bẹbẹ lọ, ati lilo awọn sẹẹli Vero, Hela, ati bẹbẹ lọ fun ipinya ọlọjẹ ati aṣa. Ṣiṣawari serological pẹlu wiwa Serum pato awọn ọlọjẹ IgM nipasẹ gbigba ELISA, ati ṣiṣawari Serum pato awọn ọlọjẹ IgG nipasẹ ELISA, immunofluorescence, ati bẹbẹ lọ.
Imọ paramita
| Ibi ipamọ | ≤30℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | omi ara, plaawọn ayẹwo sma |
| CV | ≤5.0% |
| LoD | 500 idaako / μL |
| Awọn ohun elo ti o wulo | O wulo lati tẹ reagent iwari I: Ohun elo Biosystems 7500 Real-akoko PCR Systems, QuantStudio®5 Awọn ọna PCR akoko gidi, SLAN-96P Awọn ọna PCR Akoko-gidi (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Awọn ọna Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer), MA-6000 Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Wulo fun iru II reagent iwari: EudemonTMAIO800 (HWTS-EQ007) nipasẹ Jiangsu Makiro & Micro-Test Med-Tech Co., Ltd. |
Sisan iṣẹ
Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006). Awọn isediwon yẹ ki o wa ni o waiye ni ibamu si awọn ilana, ati awọn jade awọn ayẹwo iwọn didun jẹ 200μL ati awọn niyanju elution iwọn didun ni 80μL.
