Staphylococcus Aureus ati Staphylococcus Aureus Resistant Meticcillin (MRSA/SA)

Apejuwe kukuru:

A lo ohun elo yii fun wiwa agbara ti staphylococcus aureus ati staphylococcus aureus nucleic acids-sooro methicillin ninu awọn ayẹwo sputum eniyan, awọn ayẹwo swab imu ati awọ ara ati awọn ayẹwo ikolu ti àsopọ rirọ ni fitiro.


Alaye ọja

ọja Tags

Orukọ ọja

HWTS-OT062 Staphylococcus Aureus ati Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Ohun elo Iwari Acid Nucleic (Fluorescence PCR)

Iwe-ẹri

CE

Arun-arun

Staphylococcus aureus jẹ ọkan ninu awọn kokoro arun pathogenic pataki ti ikolu nosocomial.Staphylococcus aureus (SA) jẹ ti staphylococcus ati pe o jẹ aṣoju ti awọn kokoro arun Gram-positive, eyiti o le gbe awọn oriṣiriṣi majele ati awọn enzymu apanirun.Awọn kokoro arun ni awọn abuda ti pinpin jakejado, pathogenicity ti o lagbara ati oṣuwọn resistance giga.Jiini nuclease thermostable (nuc) jẹ jiini ti o tọju pupọ ti staphylococcus aureus.

ikanni

FAM Jiini mecA-sooro meticillin
ROX

Iṣakoso ti abẹnu

CY5 staphylococcus aureus nuc jiini

Imọ paramita

Ibi ipamọ ≤-18 ℃ & aabo lati ina
Selifu-aye 12 osu
Apeere Iru sputum, awọ ara ati awọn ayẹwo àkóràn àsopọ rirọ, ati awọn ayẹwo swab imu
Ct ≤36
CV ≤5.0%
LoD 1000 CFU/ml staphylococcus aureus, 1000 CFU/mL kokoro arun meticillin-sooro.Nigbati ohun elo naa ba ṣe awari itọkasi LoD ti orilẹ-ede, 1000/mL staphylococcus aureus le ṣee rii
Ni pato Idanwo ifaseyin-agbelebu fihan pe ohun elo yii ko ni ifasilẹ agbelebu pẹlu awọn aarun atẹgun miiran bii methicillin-sensitive staphylococcus aureus, staphylococcus coagulase-negative staphylococcus, staphylococcus epidermidis-sooro methicillin, pseudomonas aeruginosa, escherichia coli, pseudomonas aeruginosa, escherichia coli, kleetobsinia. mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae.
Awọn ohun elo ti o wulo Applied Biosystems 7500 Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR eto

LineGene 9600 Plus Real-Time PCR erin System

MA-6000 Real-Time Quantitative Gbona Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Sisan iṣẹ

Aṣayan 1.

Makiro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) nipasẹ Jiangsu Makiro & Micro-Test Med-Tech Co., Ltd. le ṣee lo pẹlu Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Ṣafikun 200µL ti iyọ deede si ojoro ti a ti ṣiṣẹ, ati pe awọn igbesẹ ti o tẹle yẹ ki o fa jade ni ibamu si awọn ilana, ati iwọn didun elution ti a ṣeduro jẹ 80µL.

Aṣayan 2.

Macro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Fi 1mL ti saline deede si itọlẹ lẹhin fifọ pẹlu iyọ deede, lẹhinna dapọ daradara.Centrifuge ni 13,000r/min fun awọn iṣẹju 5, yọkuro supernatant (fipamọ 10-20µL ti supernatant), ki o tẹle awọn ilana fun isediwon atẹle.

Aṣeyọri isediwon ti a ṣe iṣeduro: Iyọkuro Acid Nucleic tabi Reagent Mimọ (YDP302) nipasẹ Tiangen Biotech (Beijing) Co., Ltd.Iyọkuro yẹ ki o ṣe ni muna ni ibamu si igbesẹ 2 ti itọnisọna itọnisọna.A gba ọ niyanju lati lo RNase ati omi ti ko ni DNase fun elution pẹlu iwọn 100µL.

 


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