Eniyan TEL-AML1 Fusion Gene Iyipada
Orukọ ọja
HWTS-TM016 Eniyan TEL-AML1 Apo Iwari Iyipada Gene (Fluorescence PCR)
Arun-arun
Lukimia lymphoblastic nla (GBOGBO) jẹ aiṣedeede ti o wọpọ julọ ni igba ewe.Ni awọn ọdun aipẹ, aisan lukimia nla (AL) ti yipada lati iru MIC (morphology, immunology, cytogenetics) si iru MICM (afikun idanwo isedale molikula).Ni ọdun 1994, a ṣe awari pe idapọ TEL ni igba ewe jẹ nitori iyipada chromosomal ti kii ṣe aibikita t(12;21)(p13;q22) ni idile B-lineage lymphoblastic leukemia (GBOGBO).Niwọn igba ti a ti ṣe awari Jiini idapọ AML1, Jiini idapọ TEL-AML1 jẹ ọna ti o dara julọ lati ṣe idajọ asọtẹlẹ ti awọn ọmọde ti o ni aisan lukimia lymphoblastic nla.
ikanni
FAM | Jiini idapọ TEL-AML1 |
ROX | Iṣakoso ti abẹnu |
Imọ paramita
Ibi ipamọ | ≤-18℃ |
Selifu-aye | osu 9 |
Apeere Iru | ọra inu egungun ayẹwo |
Ct | ≤40 |
CV | <5.0% |
LoD | 1000 Awọn ẹda/ml |
Ni pato | Ko si ifasilẹ-agbelebu laarin awọn ohun elo ati awọn jiini idapọ miiran bii BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, awọn jiini idapọ PML-RARa. |
Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
RNAprep Pure Ẹjẹ Apapọ RNA isediwon Apo (DP433).