Eniyan TEL-AML1 Fusion Gene Iyipada
Orukọ ọja
HWTS-TM016 Eniyan TEL-AML1 Apo Iwari Iyipada Gene (Fluorescence PCR)
Arun-arun
Lukimia lymphoblastic nla (GBOGBO) jẹ aiṣedeede ti o wọpọ julọ ni igba ewe. Ni awọn ọdun aipẹ, aisan lukimia nla (AL) ti yipada lati iru MIC (morphology, immunology, cytogenetics) si iru MICM (afikun idanwo isedale molikula). Ni ọdun 1994, a ṣe awari pe idapọ TEL ni igba ewe jẹ nitori iyipada chromosomal ti kii ṣe aibikita t(12;21)(p13;q22) ni idile B-lineage lymphoblastic leukemia (GBOGBO). Niwọn igba ti a ti ṣe awari Jiini idapọ AML1, Jiini idapọ TEL-AML1 jẹ ọna ti o dara julọ lati ṣe idajọ asọtẹlẹ ti awọn ọmọde ti o ni aisan lukimia lymphoblastic nla.
ikanni
| FAM | Jiini idapọ TEL-AML1 |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | ọra inu egungun ayẹwo |
| Ct | ≤40 |
| CV | <5.0% |
| LoD | 1000 Awọn ẹda/ml |
| Ni pato | Ko si ifasilẹ-agbelebu laarin awọn ohun elo ati awọn jiini idapọ miiran bii BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, awọn jiini idapọ PML-RARa. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
RNAprep Pure Ẹjẹ Total RNA isediwon Apo (DP433).
