Aarun ayọkẹlẹ A/B
Orukọ ọja
HWTS-RT003A Aarun ayọkẹlẹ A/B Ohun elo Iwari Acid Nucleic Acid (Fluorescence PCR)
Arun-arun
Kokoro aarun ayọkẹlẹ jẹ arun aarun atẹgun nla, pẹlu ọpọlọpọ awọn ipin bii H1N1 ati H3N2, eyiti o ni itara si iyipada ti o si jade kaakiri agbaye.Iyipada Antigenic tọka si iyipada ti ọlọjẹ aarun ayọkẹlẹ A, ti o yọrisi ifarahan ti subtype tuntun kan.Awọn ọlọjẹ aarun ayọkẹlẹ B ti pin si awọn idile pataki meji, Yamagata ati Victoria.Awọn ọlọjẹ aarun ayọkẹlẹ B nikan ni fiseete antigenic, ati pe wọn yago fun iwo-kakiri ati imukuro eto ajẹsara eniyan nipasẹ awọn iyipada wọn.Sibẹsibẹ, oṣuwọn itankalẹ ti aarun ayọkẹlẹ B jẹ o lọra ju ti aarun ayọkẹlẹ A, ati ọlọjẹ B tun le fa ikolu ti atẹgun eniyan ati ja si awọn ajakale-arun.
ikanni
| FAM | IFV A |
| ROX | Iṣakoso ti abẹnu |
| VIC/HEX | IFV B |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | oropharyngeal swab |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | IFV A: 500 Awọn ẹda/ml, IFV B: 500 Awọn adakọ/ml |
| Ni pato | 1. Cross-reactivity: ko si ifaseyin agbelebu laarin ohun elo yii ati iru adenovirus 3, 7, coronavirus eniyan SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, ati HCoV-NL63, cytomegalovirus, enterovirus, parainfluenza virus, measles virus, metapneumovirus eniyan, ọlọjẹ mumps, ọlọjẹ syncytial ti atẹgun iru B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacilrlenthabacterium myaxosis, pneumonia, pneumonia, pneumonia. e, neisseria meningitidis , neisseria gonorrhoeae, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, streptococcus salivarius ati DNA genomic eniyan. 2. Idanwo kikọlu: Awọn nkan ti o ni idiwọ ni a yan bi mucin (60mg / mL), ẹjẹ eniyan, oxymetazoline (2mg / mL), sulfur (10%), beclomethasone (20mg / mL), dexamethasone (20mg / mL), flunisolide ( 20μg/ml), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL) ), azithromycin (1mg/L), cephalosporin (40μg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir fosifeti (60ng/mL), ribavirin (10mg/L), ati awọn esi fihan pe awọn oludoti idilọwọ pẹlu awọn ifọkansi ti o wa loke ko ni ifọkansi kikọlu si wiwa ohun elo naa. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Awọn ọna PCR-gidi-gidi(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Eto Wiwa PCR Akoko-gidi (FQD-96A, imọ-ẹrọ Hangzhou Bioer) MA-6000 Gidigidi Gidigidi Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Eto PCR akoko-gidi ati BioRad CFX Opus 96 Eto PCR akoko-gidi |
Sisan iṣẹ
Aṣayan 1.
Niyanju isediwon reagents: Makiro & Micro-Idanwo Gbogbogbo DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Igbeyewo. Aifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. O yẹ ki o ṣe isediwon ni ibamu si IFU.Iwọn ayẹwo isediwon jẹ200μL.Iwọn elution ti a ṣe iṣeduro jẹ 80μL.
Aṣayan 2.
Niyanju isediwon reagents: Makiro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8).Iyọkuro yẹ ki o ṣe ni ibamu si IFU.
Aṣayan 3.
Niyanju isediwon reagents: Nucleic Acid isediwon tabi ìwẹnu Apo (YDP315-R).Iyọkuro yẹ ki o ṣe ni ibamu si IFU.Iwọn ayẹwo isediwon jẹ 140μL.Iwọn didun elution ti a ṣe iṣeduro jẹ 60μL.
