Mycobacterium Tuberculosis Isoniazid Resistance Mutation

Mycobacterium Tuberculosis Isoniazid Resistance Iyipada Afihan Aworan

Apejuwe kukuru:

Ohun elo yii dara fun wiwa didara ti awọn aaye iyipada akọkọ ninu awọn ayẹwo sputum eniyan ti a gba lati ọdọ awọn alaisan Tubercle bacillus rere ti o yorisi ikọlu mycobacterium isoniazid resistance: Agbegbe olupolowo InhA -15C>T, -8T>A, -8T>C;AhpC agbegbe olupolowo -12C>T, -6G>A;Iyipada homozygous ti KatG 315 codon 315G>A, 315G>C.

Fi imeeli ranṣẹ si wa

Alaye ọja

ọja Tags

Orukọ ọja

HWTS-RT137 Mycobacterium Tuberculosis Isoniazid Resistance Mutation Detection Kit (Ibi Irọ)

Arun-arun

Ikọ-ẹ̀gbẹ Mycobacterium, laipẹ bi Tubercle bacillus (TB), jẹ kokoro arun alamọja ti o fa iko.Lọwọlọwọ, awọn oogun egboogi-egboogi-ila akọkọ ti a nlo nigbagbogbo pẹlu isoniazid, rifampicin ati hexambutol, ati bẹbẹ lọ. Sibẹsibẹ, nitori lilo ti ko tọ ti awọn oogun egboogi-ikọ-ara ati awọn abuda ti eto ogiri sẹẹli ti iko-ara mycobacterium, iko-ara mycobacterium ndagba resistance oogun si awọn oogun ikọ-ara, eyiti o mu awọn italaya pataki si idena ati itọju ikọ-igbẹ.

ikanni

FAM	MP nucleic acid
ROX	Iṣakoso ti abẹnu

Imọ paramita

Ibi ipamọ	≤-18℃
Selifu-aye	12 osu
Apeere Iru	sputum
CV	≤5%
LoD	Iwọn wiwa fun awọn kokoro arun isoniazid iru-igi jẹ 2x103 kokoro arun/mL, ati pe opin wiwa fun kokoro arun mutant jẹ 2x103 kokoro arun/mL.
Ni pato	a.Ko si ifaseyin agbelebu laarin jiini eniyan, awọn mycobacteria nontuberculous miiran ati awọn ọlọjẹ pneumonia ti a rii nipasẹ ohun elo yii.b.Awọn aaye iyipada ti awọn jiini sooro oogun miiran ninu iru-igbẹ Mycobacterium iko, gẹgẹbi agbegbe ti npinnu resistance ti jiini rifampicin rpoB, ni a rii, ati pe awọn abajade idanwo ko fihan atako si isoniazid, ti n tọka ko si ifaseyin agbelebu.
Awọn ohun elo ti o wulo	SLAN-96P Real-Time PCR SystemsBioRad CFX96 Real-Time PCR Systems LightCycler480® Real-Time PCR System

Sisan iṣẹ

Ti o ba lo Makiro & Micro-Test General DNA/RNA Kit (HWTS-3019) (eyiti o le ṣee lo pẹlu Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. fun isediwon, fi 200 kunμL ti iṣakoso odi ati ayẹwo sputum ti a ṣe ilana lati ṣe idanwo ni ọkọọkan, ati ṣafikun 10μL ti iṣakoso inu lọtọ sinu iṣakoso odi, ayẹwo sputum ti a ṣe ilana lati ṣe idanwo, ati awọn igbesẹ ti o tẹle yẹ ki o ṣe ni muna ni ibamu si awọn ilana isediwon.Iwọn ayẹwo ti o jade jẹ 200μL, ati iwọn didun elution ti a ṣeduro jẹ 100μL.