Mycobacterium Tuberculosis INH Iyipada
Orukọ ọja
HWTS-RT137 Mycobacterium Tuberculosis INH Iyipada Iyipada Apo (Ibi Iyọ)
Arun-arun
Ikọ-ẹ̀gbẹ Mycobacterium, laipẹ bi Tubercle bacillus (TB), jẹ kokoro arun alamọja ti o fa iko. Lọwọlọwọ, awọn oogun egboogi-egboogi-ila akọkọ ti a lo nigbagbogbo pẹlu INH, rifampicin ati hexambutol, ati bẹbẹ lọ. ikọ-ọgbẹ mycobacterium ndagba resistance oogun si awọn oogun atako ikọ-fèé, eyiti o mu awọn ipenija nla wa si idena ati itọju ikọ-igbẹ.
ikanni
| FAM | MP nucleic acid |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | sputum |
| CV | ≤5% |
| LoD | Iwọn wiwa fun kokoro INH iru-igi jẹ 2x103 kokoro arun/mL, ati opin wiwa fun kokoro arun mutant jẹ 2x103 kokoro arun/mL. |
| Ni pato | a. Ko si ifaseyin agbelebu laarin jiini eniyan, awọn mycobacteria nontuberculous miiran ati awọn ọlọjẹ pneumonia ti a rii nipasẹ ohun elo yii.b. Awọn aaye iyipada ti awọn jiini sooro oogun miiran ninu iru-igbẹ Mycobacterium iko, gẹgẹbi agbegbe ti npinnu resistance ti jiini rifampicin rpoB, ni a rii, ati pe awọn abajade idanwo ko fihan atako si INH, ti n tọka ko si ifaseyin agbelebu. |
| Awọn ohun elo ti o wulo | SLAN-96P Real-Time PCR SystemsBioRad CFX96 Real-Time PCR SystemsLightCycler480®Real-Time PCR System |
Sisan iṣẹ
Ti o ba lo Macro & Micro-Test General DNA / RNA Kit (HWTS-3019) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. lati ṣe idanwo ti iṣakoso odi ti 200000L fun isediwon ti iṣakoso μL ati fikun ilana 20. ni ọkọọkan, ati ṣafikun 10μL ti iṣakoso inu lọtọ si iṣakoso odi, ayẹwo sputum ti a ti ni ilọsiwaju lati ṣe idanwo, ati awọn igbesẹ ti o tẹle yẹ ki o ṣe ni muna ni ibamu si awọn ilana isediwon. Iwọn ayẹwo ti o jade jẹ 200μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 100μL.
