EasyAmp nipasẹ Makiro & Idanwo Micro—- Ohun elo Imudara Isothermal Fluorescence To šee gbe ni ibamu pẹlu LAMP/RPA/NASBA/HDA

O tayọ Performance & Jakejado elo

Easy Amp, nipasẹ awọn ọna ti isothermal nucleic acid ampilifayati wa ni ifihan pẹlu gaifamọ atikukurulenuakoko lai awọn ibeere fun ilana iyipada iwọn otutu. Nítorí náà, o nifarahanedbi awọnjulọimọ-ẹrọ ayanfẹ fun idanwo iyara nucleic acid.Laisi idiju tawọn ọna ṣiṣe gigun kẹkẹ hermal, ohun elo isothermal ti o rọrun ati to ṣee gbele ni itẹlọrun awọngbogbo lenu ilana.

Makiro & Micro-Test ti ni idagbasoke ominiraati ki o se igbekaleawọn EasyAmp,ohun elo imudara isothermal Fuluorisenti to ṣee gbefun orisirisi awọn ohun elo.

Lọwọlọwọ, awọnAmp ti o rọrun ti ni ifọwọsi lati jẹNi ibamu pẹlu ọpọlọpọ awọn ilana imudara isothermal: Loop-Mediated Isothermal Amplification (LAMP), Recombinase Polymerase Amplification (RPA/RAA/MIRA), Nucleic Acid Sequence Based Amplification (NASBA), ati Helicase Dependent Amplification (HDA).Yoo jẹ ohun elo gige-eti miiran fun idanwo iyara nucleic acid pẹlu awọn ohun elo ti o gbooro pupọ.

Imudara Isothermal Yipo-Lalaja (LAMP):Imudara ni60-65°Cpẹlu odidilenuilanapari laarin 30 iṣẹju.Wiwo aworan 1 fun imudara rẹopo:

 

olusin 1. Ilanaof ATUPA 

Atunpọ Polymerase Amudara (RPA/RAA/MIRA):Imudara ni37°C-42°Cpẹlu odidilenuilanapari ni 10-30 iṣẹju.Ri Figure 2 fun awọn oniwe-ampilifaya opo:

olusin 2. Ilanaof Recombinase Polymerase Amplification

·Amufikún ti o da lori Acid Nucleic Acid (NASBA):Imudara ni42°Cpẹlu odidilenuilanapari ni 40-120 iṣẹju.Ri Figure 3 fun awọn oniwe-ampilifaya opo:

olusin 3. Ilanaof Nucleic Acid Ọkọọkan Da ampilifaya

· Imudara Igbẹkẹle Helicase (HDA):Imudara ni65°Cpẹlu odidilenuilanapari ni 70-90 iṣẹju.Ri Figure 4 fun awọn oniwe-ampilifaya opo:

aworan 4.Ilanaof Imudara ti o gbẹkẹle helicase

· Awọn EasyAmpmu ṣiṣẹs daradara ati imudara iduroṣinṣin fun DNA ati wiwa RNA nipa lilo awọn ilana isothermal mẹrin ti a mẹnuba.O ṣaṣeyọri ifamọ imudara to awọn ẹda 200 / milimita ati ṣe idaniloju iyasọtọ giga laisi ifasilẹ-agbelebu ni imudara ibi-afẹde..(Iti a ṣe apejuwe ninu Awọn nọmba 5 si 7).

DNA erin RNA erin

Fig5.RAA / MIRA Platform

DNA erin RNA erin

Fig6.Atupa Platform

 

DNA erin RNA erin

Fig7.NASBA Platform

Awọn anfani Ọja:

Iyara

· EPIAimọ ẹrọ mọesi rereri ni 5 min.

· Rọrun isẹ pẹlu ọkan-igbese isediwonfun ohun elo lori ohun elo.

Munadoko

· Awọn modulu ominira 4 fun ibeere, idanwo igbakana ti 4 oriṣiriṣiigbeyewo irus.

Modulu kọọkan ti o lagbara lati ṣe itupalẹ awọn ayẹwo mẹrin ni igbakanna.

· Meji / Quadfluorescenceawọn ikannifun sanlalu olona-afojusun erin.

Ibamu

· Ni ibamu pẹluọpọawọn iru ẹrọ isothermal gẹgẹbi LAMP, RPA, NASBA, HDA.

· Apẹrẹ fun ga-otutu ampilifaya post kekere-otutu yiyipada transcription.

· Ṣe akanṣelelati pade awọn ibeere alabara kan pato.

Onirọrun aṣamulo

· Portable design(290×245×128mm, 2kg) fun orisirisi ohun elo senarios.

· 7-inch awọ touchscreenfun išišẹ ti o rọrun ati apejuwe abajade ti o han kedere.

· Itumọ ti gbona itẹwe fun esi lẹsẹkẹsẹ titẹ sita.

· Aṣepọ scanner fun wiwa ohun kan laifọwọyi kika.

 


Akoko ifiweranṣẹ: Oṣu kejila-22-2023