Staphylococcus Aureus ati Staphylococcus Aureus Resistant Meticcillin (MRSA/SA)
Orukọ ọja
HWTS-OT062 Staphylococcus Aureus ati Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Ohun elo Iwari Acid Nucleic (Fluorescence PCR)
Iwe-ẹri
CE
Arun-arun
Staphylococcus aureus jẹ ọkan ninu awọn kokoro arun pathogenic pataki ti ikolu nosocomial.Staphylococcus aureus (SA) jẹ ti staphylococcus ati pe o jẹ aṣoju ti awọn kokoro arun Gram-positive, eyiti o le gbe awọn oriṣiriṣi majele ati awọn enzymu apanirun.Awọn kokoro arun ni awọn abuda ti pinpin jakejado, pathogenicity ti o lagbara ati oṣuwọn resistance giga.Jiini nuclease thermostable (nuc) jẹ jiini ti o tọju pupọ ti staphylococcus aureus.
ikanni
| FAM | Jiini mecA-sooro meticillin |
| ROX | Iṣakoso ti abẹnu |
| CY5 | staphylococcus aureus nuc jiini |
Imọ paramita
| Ibi ipamọ | ≤-18 ℃ & aabo lati ina |
| Selifu-aye | 12 osu |
| Apeere Iru | sputum, awọ ara ati awọn ayẹwo àkóràn àsopọ rirọ, ati awọn ayẹwo swab imu |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/ml staphylococcus aureus, 1000 CFU/mL kokoro arun meticillin-sooro.Nigbati ohun elo naa ba ṣe awari itọkasi LoD ti orilẹ-ede, 1000/mL staphylococcus aureus le ṣee rii |
| Ni pato | Idanwo ifaseyin-agbelebu fihan pe ohun elo yii ko ni ifasilẹ agbelebu pẹlu awọn aarun atẹgun miiran bii methicillin-sensitive staphylococcus aureus, staphylococcus coagulase-negative staphylococcus, staphylococcus epidermidis-sooro methicillin, pseudomonas aeruginosa, escherichia coli, pseudomonas aeruginosa, escherichia coli, kleetobsinia. mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1.
Makiro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) nipasẹ Jiangsu Makiro & Micro-Test Med-Tech Co., Ltd. le ṣee lo pẹlu Makiro & Micro-Idanwo Aifọwọyi Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Ṣafikun 200µL ti iyọ deede si ojoro ti a ti ṣiṣẹ, ati pe awọn igbesẹ ti o tẹle yẹ ki o fa jade ni ibamu si awọn ilana, ati iwọn didun elution ti a ṣeduro jẹ 80µL.
Aṣayan 2.
Macro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Fi 1mL ti saline deede si itọlẹ lẹhin fifọ pẹlu iyọ deede, lẹhinna dapọ daradara.Centrifuge ni 13,000r/min fun awọn iṣẹju 5, yọkuro supernatant (fipamọ 10-20µL ti supernatant), ki o tẹle awọn ilana fun isediwon atẹle.
Aṣeyọri isediwon ti a ṣe iṣeduro: Iyọkuro Acid Nucleic tabi Reagent Mimọ (YDP302) nipasẹ Tiangen Biotech (Beijing) Co., Ltd.Iyọkuro yẹ ki o ṣe ni muna ni ibamu si igbesẹ 2 ti itọnisọna itọnisọna.A gba ọ niyanju lati lo RNase ati omi ti ko ni DNase fun elution pẹlu iwọn 100µL.
