Kokoro Zika
Orukọ ọja
HWTS-FE002 Apo Iwari Acid Nucleic Acid (Fluorescence PCR) Zika Virus
Iwe-ẹri
CE
Arun-arun
Kokoro Zika jẹ ti iwin Flaviviridae, jẹ ọlọjẹ RNA rere ti o ni okun-ọkan kan pẹlu iwọn ila opin ti 40-70nm. O ni apoowe kan, o ni awọn nucleotides 10794, ati koodu 3419 amino acids. Gẹgẹbi genotype, o pin si iru Afirika ati iru Asia. Arun kokoro Zika jẹ arun ajakalẹ-arun nla ti o ni opin ti ara ẹni ti o fa nipasẹ ọlọjẹ Zika, eyiti o tan kaakiri nipasẹ jijẹ ti awọn ẹfọn Aedes aegypti. Awọn ẹya ara ile-iwosan jẹ nipataki iba, sisu, arthralgia tabi conjunctivitis, ati pe o ṣọwọn apaniyan. Gẹgẹbi Ajo Agbaye ti Ilera, microcephaly ọmọ tuntun ati iṣọn Guillain-Barre (aisan Guillain-Barré) le ni nkan ṣe pẹlu akoran ọlọjẹ Zika.
ikanni
| FAM | Zika kokoro nucleic acid |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤30℃ & aabo lati ina |
| Selifu-aye | 12 osu |
| Apeere Iru | omi ara tuntun |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 idaako/ml |
| Ni pato | Lo ohun elo naa lati ṣawari awọn ayẹwo omi ara pẹlu kokoro Zika odi, ati awọn abajade jẹ odi. Awọn abajade idanwo kikọlu fihan pe nigbati ifọkansi ti bilirubin ninu omi ara ko ju 168.2μmol/ml, ifọkansi haemoglobin ti a ṣe nipasẹ hemolysis ko ju 130g/L, ifọkansi ọra ẹjẹ ko ju 65mmol/ml, apapọ ifọkansi IgG ninu omi ara ko ju 5mg/mL lọ, ko si ni ipa lori kokoro arun chikun. Kokoro Hepatitis A, ọlọjẹ Hepatitis B, ọlọjẹ Hepatitis C, ọlọjẹ Herpes, ọlọjẹ equine encephalitis Eastern, Hantavirus, Bunya virus, West Nile virus ati awọn ayẹwo omi ara eniyan ni a yan fun idanwo ifasilẹ-agbelebu, ati awọn abajade fihan pe ko si ifaseyin agbelebu laarin ohun elo yii ati awọn pathogens ti a mẹnuba loke. |
| Awọn ohun elo ti o wulo | ABI 7500 Real-Time PCR SystemsABI 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1.
QIAamp Viral RNA Mini Kit(52904), Iyọkuro Acid Nucleic tabi Reagent Iwẹnumọ (YDP315-R) nipasẹ Tiangen Biotech (Beijing) Co., Ltd.Awọn isediwonyẹ ki o fa jade ni ibamu si awọn ilana isediwon, ati iwọn didun isediwon ti a ṣe iṣeduro jẹ 140 μL ati iwọn didun elution ti a ṣe iṣeduro jẹ 60 μL.
Aṣayan 2.
Makiro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ati Makiro & Micro-Test Laifọwọyi Nucleic Acid Extractor (HWTS-3006). Awọn isediwon yẹ ki o wa jade ni ibamu si awọn ilana. Iwọn ayẹwo isediwon jẹ 200 μL, ati iwọn didun elution ti a ṣe iṣeduro jẹ 80μL.
