Enterovirus 71 (EV71)
Orukọ ọja
HWTS-EV003- Enterovirus 71 (EV71) Ohun elo Iwari Acid Nucleic (Pluorescence PCR)
Arun-arun
Arun-ẹnu-ọwọ jẹ arun aarun ti o fa nipasẹ awọn enteroviruses (EV).Lọwọlọwọ, awọn iru 108 ti serotypes ti enteroviruses ni a ti rii, eyiti o pin si awọn ẹgbẹ mẹrin: A, B, C ati D. Lara wọn, enterovirus EV71 ati CoxA16 jẹ awọn ọlọjẹ akọkọ.Arun naa maa nwaye ni awọn ọmọde labẹ ọdun 5, ati pe o le fa awọn herpes ni ọwọ, ẹsẹ, ẹnu ati awọn ẹya miiran.Nọmba kekere ti awọn ọmọde yoo dagbasoke awọn ilolu bii myocarditis, edema ẹdọforo, ati meningoencephalitis aseptic.
ikanni
| FAM | EV71 |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | osu 9 |
| Apeere Iru | Oropharyngeal swab,Herpes ito |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 idaako/ml |
| Awọn ohun elo ti o wulo | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Yara Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Awọn ọna Iwari PCR akoko-gidi MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1.
Niyanju isediwon reagent: Makiro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8), ati awọn isediwon yẹ ki o wa ni o waiye ni ibamu si awọn ilana fun lilo.Awọn ayẹwo jade ni awọn swabs oropharyngeal tabi awọn ayẹwo ito Herpes lati ọdọ awọn alaisan ti o gba lori aaye.Ṣafikun awọn swabs ti a gba sinu Macro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8) taara, vortex ati dapọ daradara, gbe ni iwọn otutu yara fun awọn iṣẹju 5, mu wọn jade lẹhinna yipada lati dapọ daradara, lati gba RNA naa ti kọọkan ayẹwo.
Aṣayan 2.
Niyanju isediwon reagent: Makiro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (eyi ti o le ṣee lo pẹlu Makiro & Micro-Igbeyewo. Extractor Nucleic Acid Acid Aifọwọyi (HWTS-3006C, HWTS-3006B)) nipasẹ Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., ati isediwon yẹ ki o waiye ni ibamu si awọn ilana fun lilo.Iwọn ayẹwo ti o jade jẹ 200µL, ati iwọn didun elution ti a ṣeduro jẹ 80µL.
Aṣayan 3.
Niyanju isediwon reagent: QIAamp Viral RNA Mini Kit (52904) nipasẹ QIAGEN tabi TIANAmp Virus DNA/RNA Kit (YDP315-R), ati awọn isediwon yẹ ki o wa ni o waiye ni muna ni ibamu pẹlu awọn ilana fun lilo.Iwọn ayẹwo ti o jade jẹ 140μL, ati iwọn didun elution ti a ṣeduro jẹ 60µL.
