Enterovirus gbogbo agbaye
Orukọ ọja
HWTS-EV001- Ohun elo Iwari Acid Nucleic Acid Agbaye (Pluorescence PCR)
Arun-arun
Arun-ẹnu-ọwọ jẹ arun aarun ti o fa nipasẹ awọn enteroviruses (EV).Lọwọlọwọ, awọn iru 108 ti serotypes ti enteroviruses ni a ti rii, eyiti o pin si awọn ẹgbẹ mẹrin: A, B, C ati D. Lara wọn, enterovirus EV71 ati CoxA16 jẹ awọn ọlọjẹ akọkọ.Arun naa maa nwaye ni awọn ọmọde labẹ ọdun 5, ati pe o le fa awọn herpes ni ọwọ, ẹsẹ, ẹnu ati awọn ẹya miiran.Nọmba kekere ti awọn ọmọde yoo dagbasoke awọn ilolu bii myocarditis, edema ẹdọforo, ati meningoencephalitis aseptic.
ikanni
| FAM | EV RNA |
| ROX | Iṣakoso ti abẹnu |
Imọ paramita
| Ibi ipamọ | ≤-18℃ |
| Selifu-aye | 12 osu |
| Apeere Iru | Oropharyngeal swab,Herpes ito |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 idaako/ml |
| Awọn ohun elo ti o wulo | Awọn eto Biosystems ti a lo 7500/7500 Awọn ọna PCR gidi-gidi-gidi, QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR eto LineGene 9600 Plus Real-Time PCR erin System MA-6000 Real-Time Quantitative Gbona Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Sisan iṣẹ
Aṣayan 1.
Ṣe iṣeduro Apo Iyọkuro: Makiro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ati Makiro & Micro-Test Laifọwọyi Acid Extractor Nucleic Acid ( HWTS-3006B, HWTS-3006C), o yẹ ki o fa jade muna ni ibamu si awọn ilana.Iwọn ayẹwo jẹ 200 μL, iwọn didun elution ti a ṣe iṣeduro jẹ 80µL.
Aṣayan 2.
Ohun elo isediwon ti a ṣe iṣeduro: Makiro & Micro-Test Ayẹwo Tu Reagent (HWTS-3005-8), o yẹ ki o fa jade ni ibamu si awọn ilana naa.
Aṣayan 3.
Ohun elo isediwon ti a ṣe iṣeduro: QIAamp Viral RNA Mini Kit (52904) tabi Iyọkuro Acid Nucleic tabi Apo Iwẹnumọ (YDP315-R), o yẹ ki o fa jade ni ibamu pẹlu awọn ilana naa.Iwọn ayẹwo jẹ 140 μL, iwọn didun elution ti a ṣe iṣeduro jẹ 60µL.
